| 奶牛乳铁蛋白基因启动子区PCR-RFLP分析与乳房炎的相关性 |
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| 引用本文： | 张利军,蔡亚非,刘庆华,宋维龙,李莲,王根林.奶牛乳铁蛋白基因启动子区PCR-RFLP分析与乳房炎的相关性[J].福建农林大学学报(自然科学版),2005,34(1):87-91. |
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| 作者姓名： | 张利军 蔡亚非 刘庆华 宋维龙 李莲 王根林 |
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| 作者单位： | 南京农业大学动物科技学院,江苏,南京,210095 |
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| 基金项目： | 国家重大奶业专项基金;江苏省科技攻关项目 |
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| 摘 要： | 采用CMT方法检测奶牛的乳房炎发病情况.筛选 90头分别设为对照组(健康奶牛)、隐性乳房炎组 (试验组Ⅰ )和临床乳房炎组(试验组Ⅱ),每组 30头.检测每头奶牛的NAGase活性,并用限制性片段长度多态性 (RFLP)分析技术,检测乳铁蛋白(Lf)基因启动子区域的RFLP多态性.结果表明:不同组别的奶牛之间NAGase活性差异显著 (P<0. 01),且Lf基因启动子区域存在RFLP多态性,说明该多态性与乳房炎存在一定关系,可能是奶牛乳房炎的一个分子标记.
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| 关 键 词： | 乳铁蛋白基因 PCRRFLP 乳房炎 NAGase活性 |
| 文章编号： | 1006-7817(2005)01-0087-05 |
| 修稿时间： | 2004年5月8日 |
Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with mastitis |
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| ZHANG Li-jun,CAI Ya-Fei,LIU Qing-hua,SONG Wei-long,LI Lian,WANG Gen-Lin.Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with mastitis[J].Journal of Fujian Agricultural and Forestry University,2005,34(1):87-91. |
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| Authors: | ZHANG Li-jun CAI Ya-Fei LIU Qing-hua SONG Wei-long LI Lian WANG Gen-Lin |
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| Abstract: | CMT was used to detect the incidence of mastitis. Ninety cows were selected and allotted into 3 groups, 30 animals in each group: control group (healthy cows), experimental groupⅠ (cows with sub-clinical mastitis) and experimental group Ⅱ (cows with clinical mastitis). N-acetyl-β-D-glucosaminidase (NAGase) activity of milk samples was detected and the relationship between cow′s mastitis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the promoter of cow lactoferrin gene was explored. The results showed that there were significantly differences in NAGase activity among groups (P<(0.01)) and polymorphism existed in the promoter of cow lactoferrin gene, which suggested that this polymorphism could be related to cow′s mastitis affection and might become a molecular marker of the mastitis. |
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| Keywords: | PCR-RFLP |
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