| 3种肠毒素基因在苏云金芽孢杆菌中的分布 |
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| 引用本文： | 黄必旺,关春鸿,邱思鑫,关雄.3种肠毒素基因在苏云金芽孢杆菌中的分布[J].福建农林大学学报(自然科学版),2005,34(3):339-343. |
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| 作者姓名： | 黄必旺 关春鸿 邱思鑫 关雄 |
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| 作者单位： | 1. 生物农药与化学生物学教育部重点实验室(福建农林大学),福建,福州,350002
2. 福建省农业科学院蔬菜研究中心,福建,福州,350013 |
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| 基金项目： | 国家高技术研究发展计划(863计划);福建省自然科学基金;福建省科技厅科研项目 |
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| 摘 要： | 采用PCR方法对引自美国俄亥俄州立大学芽孢杆菌遗传保存中心(BGSC)的45个苏云金芽孢杆菌(B t)进行了hblA、bceT及entS 3种蜡状芽孢杆菌(Bc)肠毒素基因的检测.结果表明,hblA、bceT及entS的检出率分别为60%、66.7%和44.7%.这45个准菌株涉及到44个B t亚种,说明Bc肠毒素的基因在B t中是普遍存在的,B t的安全性问题仍需继续关注.
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| 关 键 词： | 苏云金芽孢杆菌 肠毒素基因 PCR |
| 文章编号： | 1671-5470(2005)03-0339-05 |
| 收稿时间： | 2004-07-06 |
| 修稿时间： | 2004-10-09 |
Distribution of three enterotoxins genes in Bacillus thuringiensis |
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| HUANG Bi-wang,GUAN Chun-hong,QIU Si-xin,GUAN Xiong.Distribution of three enterotoxins genes in Bacillus thuringiensis[J].Journal of Fujian Agricultural and Forestry University,2005,34(3):339-343. |
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| Authors: | HUANG Bi-wang GUAN Chun-hong QIU Si-xin GUAN Xiong |
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| Institution: | Fujian Agriculture and Forestry University |
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| Abstract: | The presence of hemolysin HblA(HblA),enterotoxin BecT(BecT) and enterotoxin S(EntS) genes from 45 strains of Bacillus thuringiensis(Bt) were detected respectively by polymerase chain reaction(PCR).The results showed that 60% Bt strains contained hblA gene sequences,66.7% and 44.7% of them contained becT and entS genes sequences respectively.These Bt strains obtained from BGSC included 44 H-serotypes,44 serovars.Therefore,the enterotoxin genes proved present extensively in Bt strains,suggesting that the potential risk of Bt as a biopestcide needs more attention. |
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| Keywords: | PCR |
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