| 牦牛2个α-珠蛋白基因在染色体上排列顺序的确定 |
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| 引用本文： | 袁青妍,李齐发,张庆波,黄治国,刘振山,谢庄,殷甫路.牦牛2个α-珠蛋白基因在染色体上排列顺序的确定[J].福建农林大学学报(自然科学版),2006,35(4):384-387. |
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| 作者姓名： | 袁青妍 李齐发 张庆波 黄治国 刘振山 谢庄 殷甫路 |
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| 作者单位： | 1. 南京农业大学动物科技学院,江苏,南京,210095
2. 红松洼种畜场,河北,承德,310034 |
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| 摘 要： | 本试验扩增并克隆测定了牦牛2个α-珠蛋白基因间区域的序列.根据所测结果及人、鼠、山羊、马和黄牛的α-珠蛋白基因序列比对中的保守区设计了2对特异性引物,扩增并克隆测定了牦牛α-珠蛋白的上、下游基因序列.将测定的牦牛α-珠蛋白基因间区域序列进行同源性搜索,发现没有任何一个基因与它同源.上、下游基因的测序结果表明,上游基因为牦牛α1-珠蛋白基因,下游基因为α2-珠蛋白基因.这表明牦牛的2个α-珠蛋白基因在染色体上紧密排列,其中α1-珠蛋白基因在前,α2-珠蛋白基因在后.
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| 关 键 词： | 牦牛 α-珠蛋白基因间区域 α_1-珠蛋白基因 α_2-珠蛋白基因 |
| 文章编号： | 1671-5470(2006)04-0384-04 |
| 收稿时间： | 2005-06-15 |
| 修稿时间： | 2005-12-19 |
Confirming of the order of yak's two α-globin genes in the chromosome |
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| YUAN Qing-yan,LI Qi-fa,ZHANG Qing-bo,HUANG Zhi-guo,LIU Zhen-shan,XIE Zhuang,YIN Pu-lu.Confirming of the order of yak''''s two α-globin genes in the chromosome[J].Journal of Fujian Agricultural and Forestry University,2006,35(4):384-387. |
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| Authors: | YUAN Qing-yan LI Qi-fa ZHANG Qing-bo HUANG Zhi-guo LIU Zhen-shan XIE Zhuang YIN Pu-lu |
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| Institution: | 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;2, Hongsongwa Breeder Farm, Chengde, Hebei 310034, China |
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| Abstract: | Two differential primers were designed from the sequence of yak′s two α-globin genes′s intergenic region which was cloned and sequenced,and from the conserved region of human,mouse,goat,horse and cattle′s α-globin gene for confirming the order of yak′s two α-globin genes.There was no other gene in the intergenic region of yak′s two α-globin genes after blasting.The upstream gene was identified as yak′s α_1-globin gene,while the downstream gene was identified as yak′s α_2-globin gene after cloning and sequencing.These indicate that the yak′s two α-globin genes are located in the chromosome tightly,α_1-globin gene in the front and α_2-globin gene at the back. |
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| Keywords: | yak the intergenic region of yak′s two α-globin genes α_1-globin gene α_2-globin gene |
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