南亚实蝇成虫肠道细菌的分离与鉴定

采用传统的微生物培养和16S r DNA(V6-V8)高变区测序相结合的方法,从室内人工饲养的性成熟南亚实蝇雌虫肠道中分离、鉴定出21株细菌,均属于γ-变形菌门,分别为肠杆菌属(7株)、拉乌尔菌属(3株)、沙雷氏菌属(2株)、普罗维登斯菌属(2株)、不动杆菌属(1株)、柠檬酸杆菌属(1株)、西地西菌属(1株)和不可培养的细菌(4株);从性成熟的南亚实蝇雄虫肠道内分离得到20株细菌,分别为细菌(7株)、克雷伯氏菌属(4株)、肠杆菌属(3株)、普罗维登斯菌属(2株)、沙雷氏菌属(1株)、摩根菌属(1株)、假单胞菌属(1株)和γ-变形杆菌(1株),也均属于γ-变形菌门.由上可知,性成熟的南亚实蝇雌、雄成虫肠道共有细菌为肠杆菌属、普罗维登斯菌属和沙雷氏菌属,均属于肠杆菌科,表明肠杆菌科是南亚雌、雄成虫肠道细菌的优势菌群.

Isolation and identification of bacteria in the intestinal tract of adult Bactrocera tau (Walker) (Diptera:Tephritidae)

21 strains of bacteria were isolated and identified from the intestinal tract of sexually matured female Bactrocera tau which were reared in the laboratory using traditional isolation and 16S rDNA ( V6-V8 hypervariable region) sequencing technique. All bacteria strains belonged to the Gamma-proteobacteria phylum, which included Enterobacter (7 strains), Raoultella (3 strains), Serratia ( 2 strains) , Providencia ( 2 strains) , Acinetobacter ( 1 strain) , Citrobacter ( 1 strain) , Cedecea ( 1 strain) and uncultured bacteria (4 strains). Similarly, 20 bacteria strains were isolated and identified from male B.tau and they also belonged to Gamma-proteobacteria phylum, which consisted of bacteria (7 strains), Klebsiella (4 strains), Enterobacter (3 strains), Providencia (2 strains), Serratia (1 strain ), Morganella (1 strain), Pseudomonas (1 strain ) and gamma Proteobacterium (1 strain). Bacteria genus which both females and males had in common were Enterobacter, Providencia and Serratia, which all belonged to Enterobacte-riaceae. To summarize, Enterobacteriaceae was the dominant bacterium in intestinal tract of B.tau.