| 紫外线辐射的葡萄幼果cDNA文库的构建 |
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| 引用本文： | 夏信明,刘金仙,林明,王育娜,何水林.紫外线辐射的葡萄幼果cDNA文库的构建[J].福建农林大学学报(自然科学版),2007,36(2):151-153. |
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| 作者姓名： | 夏信明 刘金仙 林明 王育娜 何水林 |
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| 作者单位： | 福建农林大学生命科学学院,福建,福州,350002 |
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| 基金项目： | 国家自然科学基金
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福建省自然科学基金 |
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| 摘 要： | 以紫外线辐射的葡萄幼果为材料提取总RNA,用SMARTTMcDNA文库构建试剂盒构建cDNA文库,初始文库含有1.5×106个独立的单克隆,扩增后滴度达到1.2×108pfu.mL-1.随机挑取噬菌斑进行PCR检测,发现重组率在92.9%以上.
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| 关 键 词： | 葡萄 幼果 cDNA文库 紫外线辐射 |
| 文章编号： | 1671-5470(2007)02-0151-03 |
| 修稿时间： | 2006-02-16 |
The construction of cDNA library with RNA isolated from young fruit of grape challenged with ultraviolet radiation |
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| XIA Xin-ming,LIU Jin-xian,LIN Ming,WANG Yu-na,HE Shui-lin.The construction of cDNA library with RNA isolated from young fruit of grape challenged with ultraviolet radiation[J].Journal of Fujian Agricultural and Forestry University,2007,36(2):151-153. |
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| Authors: | XIA Xin-ming LIU Jin-xian LIN Ming WANG Yu-na HE Shui-lin |
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| Institution: | College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | The total RNA was isolated from young fruits of grape challenged with ultraviolet radiation,and with the isolated total RNA,a cDNA library with more than 1.5 ×106 individual clones was constructed with smarttm cDNA construction kit.The titer of the amplification cDNA library was more than 1.2×108 pfu·mL-1 and the recombinant ratio was more than 92.9%. |
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| Keywords: | grape young fruit cDNA library ultraviolet radiation |
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