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| 南方红豆杉愈伤组织诱导和继代培养体系的建立 | |
| 引用本文： | 李斌连,邱萃,刘金仙,王贵荣,何水林.南方红豆杉愈伤组织诱导和继代培养体系的建立[J].福建农林大学学报(自然科学版),2006,35(5):515-518. |
| 作者姓名： | 李斌连 邱萃 刘金仙 王贵荣 何水林 |
| 作者单位： | 1. 福建农林大学植物生物技术试验室,福建,福州,350002;徐州医药高等职业技术学校,江苏,徐州,221116 2. 福建农林大学植物生物技术试验室,福建,福州,350002 3. 中国药科大学中药学院,江苏,南京,210038 |
| 摘 要： | 以南方红豆杉为材料,建立了其愈伤组织诱导与保持的技术体系,主要为:(1)南方红豆杉侧芽是诱导愈伤组织的最佳外植体;(2)南方红豆杉愈伤组织诱导的最佳培养基为:B5+2.0 mg.L-12,4-D+1.0 mg.L-1NAA+0.2 mg.L-1IAA;(3)南方红豆杉愈伤组织增殖的培养基为:B5+4.0 mg.L-1NAA+0.5 mg.L-12,4-D+0.2 mg.L-1GA+500 mg.L-1AC+2000 mg.L-1LH.并在培养基中同时添加AC、LH和GA33种抗褐物质,取得较好的抗褐化效果. |
| 关 键 词： | 南方红豆杉 外植体 愈伤组织诱导 褐化 继代培养 |
| 文章编号： | 1671-5470(2006)05-0515-04 |
| 收稿时间： | 2005-11-15 |
| 修稿时间： | 2006-04-15 |
Establishment of callus induction and subculture system of Taxus chinensis var.mairei |
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| LI Bin-lian,QIU Cui,LIU Jin-xian,WANG Gui-rong,HE Shui-lin.Establishment of callus induction and subculture system of Taxus chinensis var.mairei[J].Journal of Fujian Agricultural and Forestry University,2006,35(5):515-518. | |
| Authors: | LI Bin-lian QIU Cui LIU Jin-xian WANG Gui-rong HE Shui-lin |
| Abstract: | The callus induction and maintenance system of Taxus chinensis var.mairei was established in the experiment,the main results were as follows:(1) lateral buds were the best explants for the callus induction;(2) B_5 plus 2.0 mg·L~(-1) 2,4-D,1.0 mg·L~(-1) NAA and 1.0 mg·L~(-1) IAA was the best medium for callus induction;(3) B′_5 containing 4.0 mg·L~(-1) NAA,0.5 mg·L~(-1) 2,4-D,(0.2) mg·L~(-1) GA_3,500 mg·L~(-1) AC and 2000 mg·L~(-1) LH was the best medium for callus growth.The brownness of callus could be avoided or decreased to a certain degree when AC,LH and GA_3 were added to the mediums. |
| Keywords: | Taxus chinensis var mairei explant callus induction brownness subculture |
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