| 银杏愈伤组织细胞生长及其黄酮苷含量 |
| |
| 引用本文： | 陈发兴,赖钟雄.银杏愈伤组织细胞生长及其黄酮苷含量[J].福建农林大学学报(自然科学版),2004,33(4):456-458. |
| |
| 作者姓名： | 陈发兴 赖钟雄 |
| |
| 作者单位： | 福建农林大学园艺植物生物工程研究所;福建农林大学亚热带果树研究所,福建,福州,350002 |
| |
| 摘 要： | 对银杏不同外植体愈伤组织生长进行研究,并应用高效液相色谱(HPLC)法测定黄酮苷含量.结果表明:银杏胚乳Ⅰ愈伤组织诱导率高达100%,其生物量最高,达893mg·瓶-1;以MS为基本培养基,添加2mg·L-16 BA、1mg·L-1NAA,有利于次生代谢物的合成和积累,黄酮苷含量高达8.7mg·g-1.
|
| 关 键 词： | 银杏 愈伤组织 高效液相色谱 黄酮苷 |
| 文章编号： | 1006-7817(2004)04-0456-03 |
| 修稿时间： | 2004年3月10日 |
The callus of Ginkgo biloba and its flavonol glycosides |
| |
| CHEN Fa-xing.The callus of Ginkgo biloba and its flavonol glycosides[J].Journal of Fujian Agricultural and Forestry University,2004,33(4):456-458. |
| |
| Authors: | CHEN Fa-xing |
| |
| Institution: | CHEN Fa-xing~ |
| |
| Abstract: | The induction of calli on different explants of Ginkgo biloba and the determination of the flavonol glycosides from its callus by high-performance liquid chromatography (HPLC) were studied. The rate of induction calli from endosperm Ⅰ was highest (100%), the biological yield of calli was up to 893 mg in each flask. The optimum concentration basic medium MS supplemented with 2 mg·L~(-1) 6-BA and 1 mg·L~(-1) NAA promoted the formation of secondary metabolites, which resulted in 8.7 mg·g~(-1) of flavonol glycosides. |
| |
| Keywords: | Ginkgo biloba callus high-performance liquid chromatography flavonol glycoside |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
