|
|||||
|
|
| 25份普通烟草种质资源遗传多样性的SSR标记分析 | |
| 引用本文： | 杨柳,汪斌,童治军,黄勇兵,曹梅秀,吴海乔,巫升鑫,陈顺辉,兰涛.25份普通烟草种质资源遗传多样性的SSR标记分析[J].福建农林大学学报(自然科学版),2013,42(2). |
| 作者姓名： | 杨柳 汪斌 童治军 黄勇兵 曹梅秀 吴海乔 巫升鑫 陈顺辉 兰涛 |
| 作者单位： | 1. 福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州,350002 2. 浙江大学农业与生物技术学院,浙江杭州,310058 3. 福建省烟草科学研究所,福建福州,350003 |
| 基金项目： | 中国烟草总公司福建省公司资助项目，福建省教育厅资助项目 |
| 摘 要： | 利用14对多态明显、条带清晰的烟草SSR引物,对25份烟草种质资源进行亲缘关系与遗传多样性分析.在14个SSR位点上,共检测到97个等位基因,平均每对引物等位基因数为6.9个.引物的多态信息量(PIC)值为0.53-0.815,鉴别能力良好.25份烟草种质间的遗传相似系数(GS)为0.701-0.959,平均值为0.787.聚类分析表明,25份普通烟草种质间的遗传差异与其品种特性、调制类型、地理来源的差异没有必然联系,但能较好地反映烟草种质间亲缘关系的远近. |
| 关 键 词： | 烟草 SSR标记 遗传多样性 亲缘关系 |
Genetic diversity analysis of 25 Nicotiana tabacum L.germplasm resources based on SSR marker |
|
| Abstract: | To investigate the genetic diversity of tobacco germplasms,25 common cultivated tobacco varieties were amplified by 14 pairs of clear and polymorphic SSR(simple sequence repeat) primers.There were 97 alleles in total,and the average value per primer was 6.9 alleles.The PIC(polymorphism information content) values of primers ranged from 0.53 to 0.815,with good identification ability.The genetic similarity coefficients(GS) among 25 tobacco germplasms were 0.701-0.959,the average value was 0.787.The result of cluster analysis indicated that there was no direct correlation between genetic difference and its variety characteristics,modulation types,geographical origins of 25 tobacco germplasms,but preferably reflected the genetic relations of cultivated tobacco germplasms. |
| Keywords: | tobacco simple sequence repeat marker genetic diversity genetic relationship |
| 本文献已被 万方数据 等数据库收录！ | |