| 稻瘟病抗性基因Pi-1连锁SSR标记的筛选和应用 |
| |
| 引用本文： | 陈志伟,官华忠,吴为人,周元昌,韩庆典.稻瘟病抗性基因Pi-1连锁SSR标记的筛选和应用[J].福建农林大学学报(自然科学版),2005,34(1):74-77. |
| |
| 作者姓名： | 陈志伟 官华忠 吴为人 周元昌 韩庆典 |
| |
| 作者单位： | 福建农林大学作物科学学院,福建,福州,350002 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划);福建省科技厅科研项目;福建省教育厅科研项目 |
| |
| 摘 要： | 根据稻瘟病抗性基因Pi 1在水稻物理图谱和水稻微卫星 (SSR)遗传图谱上的位置信息,获得在抗性供体亲本(LAC23)与受体亲本(珍汕 97B、金山B 1和金山S 1)间扩增产物表现出多态性的SSR标记MGR4766,它与Pi 1的遗传距离仅为 1. 3cM;分子标记辅助选择选择准确性验证表明:RM224对Pi 1选择的准确率可达 90%以上,而MRG4766对Pi 1选择的准确率为 98% -100%;同时利用RM224和MRG4766对Pi 1选择的准确率高达 100%.
|
| 关 键 词： | 分子标记辅助选择 稻瘟病 Pi1 抗病育种 |
| 文章编号： | 1006-7817(2005)01-0074-04 |
| 修稿时间： | 2004年11月5日 |
The screening of molecular markers closely linked to rice blast resistant gene Pi-1 and their application |
| |
| CHEN Zhi-Wei,GUAN Hua-zhong,WU Wei-ren,ZHOU Yuan-chang,HAN Qing-dian.The screening of molecular markers closely linked to rice blast resistant gene Pi-1 and their application[J].Journal of Fujian Agricultural and Forestry University,2005,34(1):74-77. |
| |
| Authors: | CHEN Zhi-Wei GUAN Hua-zhong WU Wei-ren ZHOU Yuan-chang HAN Qing-dian |
| |
| Abstract: | According to the published position information of Pi-1 gene on the physical map and the SSR genetic map of rice, a SSR markers MRG4766,which showed polymorphism among resistant donor parent (LAC23) and recipient parents (Zhenshan 97B, Jinshan B-1 and Jinshan S-1), was obtained. The marker were closely linked to the rice blast-resistant gene Pi-1 with a distance of (1.3) cM. The validation of correctness rate of marker-assisted selection indicated, the correctness rate of selection for Pi-1 using SSR marker RM224 was over 90%, and that of MRG4766 was 98% to 100%. The correctness rate could reach 100% when RM224 and MRG4766 were used simultaneously. |
| |
| Keywords: | Pi-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
