蜡蚧轮枝菌对烟粉虱的时间-剂量-死亡率模型研究 |
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引用本文: | 王联德,黄建,林谷园,梁智生.蜡蚧轮枝菌对烟粉虱的时间-剂量-死亡率模型研究[J].福建农林大学学报(自然科学版),2003,32(2):167-171. |
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作者姓名: | 王联德 黄建 林谷园 梁智生 |
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作者单位: | 福建农林大学植物保护学院,福建,福州,350002 |
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基金项目: | 国家自然科学基金资助项目(39970511、30270904). |
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摘 要: | 以叶片浸液接种法测定了烟粉虱对蜡蚧轮枝菌菌株(Vp28和Vl6063)的感染反应.运用时间、剂量、死亡率模型对死亡率随时间和剂量的变化作了拟合.接种后4-8d,Vl6063的LC50分别为3.98×108,3.55×107、6.31×106、1.17×106、6.03×105孢子·mL-1;Vp28的LC50分别为6.61×106,1.95×106、1.10×106、7.76×105和6.03×105孢子·L-1.2个菌株的LT50值为105-106孢子·mL-1(约为5.5-10.0d).在小于105孢子·mL-1的剂量下,Vp28的LT50值大于Vl6063;在大于106孢子·mL-1剂量下,Vl6063的LT50值则大于Vp28、Vp28的.
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关 键 词: | 蜡蚧轮枝菌 烟粉虱 生物测定 时间-剂量-死亡率模型 |
文章编号: | 1006-7817(2003)02-0167-05 |
修稿时间: | 2002年9月30日 |
The time-dose-mortality model of two Verticillium lecanii isolates on sweetpotato whitefly,Bemisia tabaci |
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Abstract: | Bioassays of 2 isolates (Vp28, Vl6063) of Verticillium lecanii (Zimm.) were conducted on Bemisia tabaci. For inoculation, batches of 3rd instar whitefly nymphs on sweet potato seedlings were immersed in conidial suspensions at the content of 103-107 conidia·mL-1. The nymphs were maintained at 25 ℃, under 90% RH, photo phase of 16∶8 (L∶D) and observed daily for mortality. The resulting time dose mortality data fitted well to a conditional mortality probability model based on the Hosmer Lemeshow test, resulting in a cumulative mortality probability model. The parameters from the latter model were used to estimate the lethal concentration (LC50) and time (LT50) for two isolates against Bemisia tabaci. The LC50 values for Vl6063 were 3.98×108,3.55×107,6.31×106,1.17×106,6.03×105 conidia·mL-1 on 4-8 d after inoculation, and the values for Vp28 were 6.61×106,1.95×106,1.10×106,7.76×105,6.03×105 conidia·mL-1 on 4-8 d after inoculation, respectively. The estimates of LT50 values were similar at the dosage of 105-106 conidia·mL-1 for two isolates. The results indicated that isolate Vl6063 from Canada was also a promising microbial agent as isolate Vp28 for whiteflies in Fuzhou, China. |
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Keywords: | Verticillium lecanii Bemisia tabaci bioassay time-dose-mortality model |
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