水稻苗期耐盐性基因SST分子标记的筛选与应用

以水稻耐盐突变体sst为对照,对67个水稻亲本及突变体sst与水稻品种华占的F2群体进行耐盐性筛选,并结合分子标记结果,分析水稻苗期耐盐性基因SST分子标记ID27093、ID27101、ID27118、INDEL1和INDEL2的特异性和准确性.结果表明,分子标记ID27093、ID27101、ID27118和INDEL1的扩增多态性较低;而分子标记INDEL2的扩增多态性较高,为共显性标记,具有较高的特异性,能区分耐盐供体sst与85.07%的供试亲本,且筛选准确率高.因此,分子标记INDEL2标记可以直接用于耐盐性基因SST分子标记辅助育种.

Screening and application of molecular marker for salt tolerance gene SST at rice seedling stage

Molecular markers, INDEL1 and INDEL2, which are linked to salt tolerance gene SST of rice seedling, were designed. The specificity and reliability of 5 markers, including ID27093, ID27101, ID27118, INDEL1, and INDEL2, were analyzed through screening 67 rice varieties and a F2 population from a cross between mutant sst and a rice restorer line Huazhan for salt tolerance, and mutant sst was used as a salt tolerance control. The results showed that all markers except INDEL2 demonstrated low polymor-phism. INDEL2 turned out to be a co-dominant marker with high specificity, and it was able to distinguish from 85.07% of the tested parents and mutant sst with high screening accuracy. Therefore, marker INDEL2 could be directly used as a molecular marker in breeding for rice seedling salt tolerance gene SST.