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胁迫中华蜜蜂幼虫肠道的球囊菌及其体外培养的高表达基因分析
引用本文:陈大福,王鸿权,李汶东,熊翠玲,郑燕珍,付中民,徐细建,黄枳腱,郭睿.胁迫中华蜜蜂幼虫肠道的球囊菌及其体外培养的高表达基因分析[J].福建农林大学学报(自然科学版),2017,46(5).
作者姓名:陈大福  王鸿权  李汶东  熊翠玲  郑燕珍  付中民  徐细建  黄枳腱  郭睿
作者单位:福建农林大学蜂学学院,福建 福州,350002
基金项目:现代农业产业技术体系建设专项资金,福建省中青年教师教育科研项目,福建农林大学科技发展基金,国家级大学生创新创业项目
摘    要:利用RNA-seq技术对胁迫中华蜜蜂(简称中蜂)6日龄幼虫肠道的球囊菌及其体外培养进行深度测序,根据基因的FPKM值筛选得到高表达基因(HEGs),进而对HEGs进行GO及KEGG代谢通路(pathway)富集分析.Illumina测序数据经质控和过滤得到100814558条有效读段(clean reads),平均Q30均在93.81%以上.GO富集分析结果显示处理组(Aac T)的HEGs富集于39个GO term,基因富集数最多的是细胞(1872 unigene)、细胞组件(1872 unigene)和细胞进程(1748 unigenes);对照组(Aa CK)的HEGs富集于37个GO term,基因富集数最多的是细胞(785 unigenes),其次是细胞组件(785 unigenes)和代谢进程(776 unigenes).KEGG pathway富集分析显示,Aac T的HEGs富集在119个代谢通路上,基因富集数最多的是核糖体(176 unigenes)、碳代谢(147 unigenes)及氨基酸的生物合成(134 unigenes);Aa CK的HEGs富集在110个代谢通路上,基因富集数最多的是核糖体(179 unigenes)、氨基酸的生物合成(70 unigenes)以及碳代谢(62 unigenes).深入分析发现,对于富集在MAPK信号通路上的高表达基因数量,胁迫中蜂幼虫肠道的球囊菌远多于体外培养的球囊菌,说明病原的该通路在胁迫后期被显著激活.

关 键 词:中华蜜蜂  RNA-seq  球囊菌  高表达基因

Analysis of highly expressed genes of Ascosphaera apis infecting the gut of Apis cerana cerana larvae and its in vitro culture
CHEN Dafu,WANG Hongquan,LI Wendong,XIONG Cuiling,ZHENG Yanzhen,FU Zhongmin,XU Xijian,HUANG Zhijian,GUO Rui.Analysis of highly expressed genes of Ascosphaera apis infecting the gut of Apis cerana cerana larvae and its in vitro culture[J].Journal of Fujian Agricultural and Forestry University,2017,46(5).
Authors:CHEN Dafu  WANG Hongquan  LI Wendong  XIONG Cuiling  ZHENG Yanzhen  FU Zhongmin  XU Xijian  HUANG Zhijian  GUO Rui
Abstract:In this study, Ascosphaera apis spores (AacT), from the gut of 6-day-old Apis cerana cerana larvae, and A.c.cerana were sequenced utilizing RNA-seq technology. Pure A. apis spore was used as the control ( AaCK) . A total of 100814558 clean reads with a mean Q30 of 93.81% were obtained after quality control and filtration. GO enrichment analysis suggested that the highly ex-pressed genes ( HEGs) in AacT were involved in 39 terms, which was mostly enriched in cell ( 1872 unigenes) , followed by cellular component ( 1872 unigenes) and cellular process ( 1748 unigenes) . And the HEGs in AaCK were engaged in 37 terms, with the lar-gest group in cell ( 785 unigenes) , followed by cellular component ( 785 unigenes) and metabolic process ( 776 unigenes) . KEGG enrichment analysis showed that the HEGs in AacT were enriched in 119 pathways, dominating in ribosme (176 unigenes), fol-lowed by carbon metabolism (147 unigenes) and biosynthesis of amino acids (134 unigenes). While the HEGs in AaCK were in-volved in 119 pathways, and the largest groups were ribosome ( 179 unigenes) , biosynthesis of amino acids ( 70 unigenes) and car-bon metabolism ( 62 unigenes) .
Keywords:Apis cerana cerana  RNA-seq  Ascosphaera apis  highly expressed genes
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