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| 一株降解纤维素嗜热真菌的筛选、鉴定及其产酶条件的优化 | |
| 引用本文： | 余能富,吴祖建,黄一帆,刘斌.一株降解纤维素嗜热真菌的筛选、鉴定及其产酶条件的优化[J].福建农林大学学报(自然科学版),2011,40(6):636-641. |
| 作者姓名： | 余能富 吴祖建 黄一帆 刘斌 |
| 作者单位： | 1. 福建农林大学植物病毒研究所,福建福州350002;江西省林业科学院,江西南昌330032 2. 福建农林大学植物病毒研究所,福建福州,350002 3. 福建农林大学食品科学学院,福建福州,350002 4. 福建农林大学植物病毒研究所,福建福州350002;福建农林大学食品科学学院,福建福州350002 |
| 基金项目： | 国家自然科学基金，福建省校企合作重大资助项目 |
| 摘 要： | 以羧甲基纤维素钠为唯一碳源,从福州永泰温泉分离到1株降解纤维素的嗜热菌YT-40-1,经过形态观察和ITS rD-NA序列比较分析,初步鉴定为近平滑假丝酵母(Candida parapsilosis).菌株YT-40-1在初始pH为4.0、温度为50℃、接种量为10％、摇床转速为200 r·min-1的条件下在培养基中... |
| 关 键 词： | 降解纤维素 嗜热菌 产酶条件 |
Screening and identification of a thermophilic celluolytic fungi and its optimization of celluase production |
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| YU Neng-fu,WU Zu-jian,HUANG Yi-fan,LIU Bin.Screening and identification of a thermophilic celluolytic fungi and its optimization of celluase production[J].Journal of Fujian Agricultural and Forestry University,2011,40(6):636-641. | |
| Authors: | YU Neng-fu WU Zu-jian HUANG Yi-fan LIU Bin |
| Abstract: | Using sodium carboxymethyl cellulose as sole carbon resource,a thermophile fungi YT-40-1 degrading cellulose was isolated from hot spring of Yongtai County in Fuzhou.Based on rDNA sequence analysis,the strain was identified as Candida parapsilosis.The enzyme yield of strain YT-40-1 reached its peak after culturing 72 h under the conditions as follows: initial pH was 4.0,temperature was 50 ℃,inoculum was 10%,rotation speed was 200 r·min-1. |
| Keywords: | cellulose degration thermophile condition of celluase production |
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