| 金色链霉菌接合转移体系的构建 |
| |
| 引用本文： | 方志锴,洪文荣,严凌斌,潘成奇,朱宝泉.金色链霉菌接合转移体系的构建[J].福建农林大学学报(自然科学版),2011,40(5):521-524. |
| |
| 作者姓名： | 方志锴 洪文荣 严凌斌 潘成奇 朱宝泉 |
| |
| 作者单位： | 1. 福州大学生物科学与工程学院,福建福州,350108
2. 上海医药工业研究院,上海,200040 |
| |
| 摘 要： | 以金色链霉菌J13基因组DNA为模板,扩增金霉素C6甲基化酶基因ctcF上下游序列作为两端同源交换臂,在两臂之间添加抗性筛选标记neo基因,并在该基因上游加入组成型强启动子ermE*,以强化筛选标记.将该外源DNA序列插入到质粒pSET152,构建重组质粒pFD106.转化大肠杆菌ET12567后,经接合转移导入金色链...
|
| 关 键 词： | 金色链霉菌 去甲基金霉素 接合转移 |
Construction of the conjugal transfer system of Streptomyces aureofaciens |
| |
| FANG Zhi-kai,HONG Wen-rong,YAN Ling-bin,PAN Cheng-qi,ZHU Bao-quan.Construction of the conjugal transfer system of Streptomyces aureofaciens[J].Journal of Fujian Agricultural and Forestry University,2011,40(5):521-524. |
| |
| Authors: | FANG Zhi-kai HONG Wen-rong YAN Ling-bin PAN Cheng-qi ZHU Bao-quan |
| |
| Abstract: | Two exchange arms,which were respectively located upstream and downstream of gene ctcF encoding methylase of the C6 position of chlortetracycline,were amplified by PCR from Streptomyces aureofaciens J13 genomic DNA.The marker gene neo and constitutive promoter ermE* were then inserted between the arms.With these fragments,we constructed pSET152-derived vector pNEO152.The recombinant vector was transformed to Escherichia coli ET12567(pUZ8002),and then transfered to S.aureofaciens J13 by conjugation.The positive transformants were selected on MS plate.After PCR identification,it was confirmed that pFD106 was integrated into the chromosome. |
| |
| Keywords: | Streptomyces aureofaciens demeclocycline conjugation |
| 本文献已被 万方数据 等数据库收录！ |
|
