| 苏云金芽胞杆菌BRC-HZP7杀虫晶体蛋白多样性分析 |
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| 引用本文： | 沙莉,章淘,黄张敏,黄志鹏,关雄.苏云金芽胞杆菌BRC-HZP7杀虫晶体蛋白多样性分析[J].福建农林大学学报(自然科学版),2011,40(5):516-520. |
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| 作者姓名： | 沙莉 章淘 黄张敏 黄志鹏 关雄 |
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| 作者单位： | 福建农林大学生物农药与化学生物学教育部重点实验室,福建福州,350002 |
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| 摘 要： | 分离自武夷山土壤的苏云金芽胞杆菌BRC-HZP7菌株对小菜蛾和甜菜夜蛾幼虫有较好的毒杀作用.对该菌株进行了生理生化测定、cry基因的PCR-RFLP检测,以及通过SDS-PAGE检测晶体蛋白,并对蛋白点进行肽段指纹图谱鉴定.结果表明该菌株含有cry1Aa、cry1 Ba、cry1 Ia、cry2Ab、cry2Ac、cr...
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| 关 键 词： | 苏云金芽胞杆菌 PCR-RFLP cry基因 杀虫晶体蛋白 肽段指纹图谱 |
ICPs polymorphism of Bacillus thuringiensis BRC-HZP7 |
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| SHA Li,ZHANG Tao,HUANG Zhang-min,HUANG Zhi-peng,GUAN Xiong.ICPs polymorphism of Bacillus thuringiensis BRC-HZP7[J].Journal of Fujian Agricultural and Forestry University,2011,40(5):516-520. |
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| Authors: | SHA Li ZHANG Tao HUANG Zhang-min HUANG Zhi-peng GUAN Xiong |
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| Abstract: | Bacillus thuringiensis strain BRC-HZP7 was isolated from the soil of Wuyi mountain,which exhibited the high activity against Plutella xylostella larvae and Spodoptera exigua larvae.Biochemical reactions were tested for its biological characters.The cry gene types and their encoded proteins of BRC-HZP7 were identified by the methods of PCR-RFLP,SDS-PAGE and PMF.The results showed that cry1Aa,cry1Ba,cry1Ia,cry2Ab,cry2Ac and cry2Ae existed in BRC-HZP7.Eight interesting protein bands were picked out to analyze further by MALDI-TOF/MS.PMF matching analysis results showed that band 3,6,7,8 were ICPs.The band 6,7,8 were identified as Cry2Aa,Cry1Aa,Cry1Ba,respectively.The band 3 was possible partial fragment of Cry1Ba.The strain with abundant ICPs would be a good resource in pest biocontrol. |
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| Keywords: | Bacillus thuringiensis PCR-RFLP cry gene insecticidal crystal proteins peptide mass footprint |
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