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| ISSR标记在茶树品种遗传多态性研究中的应用 | |
| 引用本文： | 唐玉海,郭春芳,张木清,陈常颂,陈荣冰.ISSR标记在茶树品种遗传多态性研究中的应用[J].福建农林大学学报(自然科学版),2007,36(1):51-55. |
| 作者姓名： | 唐玉海 郭春芳 张木清 陈常颂 陈荣冰 |
| 作者单位： | 1. 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002 2. 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002;福建教育学院,福建,福州,350025 3. 福建省农业科学院茶叶研究所,福建,福安,355015 |
| 基金项目： | 福建省自然科学基金 , 福建省教育厅科研项目 |
| 摘 要： | 用筛选出的12个ISSR引物对17个茶树品种的基因组DNA进行扩增,共扩增出61个条带,其中多态性条带53条,占87.08%,平均每个引物组合扩增出5.08个条带.根据Nei-Li系数将17个茶树品种聚为2类.结果显示,ISSR是一种重复性好、效率高的分子标记,可以用于茶树品种的遗传多态性分析. |
| 关 键 词： | ISSR 茶树 聚类分析 分子标记 |
| 文章编号： | 1671-5470(2007)01-0051-05 |
| 修稿时间： | 2006-11-20 |
Application of ISSR markers to the genetic polymorphism of Camellia sinensis |
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| TANG Yu-hai,GUO Chun-fang,ZHANG Mu-qing,CHEN Chang-song,CHEN Rong-bing.Application of ISSR markers to the genetic polymorphism of Camellia sinensis[J].Journal of Fujian Agricultural and Forestry University,2007,36(1):51-55. | |
| Authors: | TANG Yu-hai GUO Chun-fang ZHANG Mu-qing CHEN Chang-song CHEN Rong-bing |
| Abstract: | The application of ISSR to the relationship analysis of tea cultivars(Camellia sinensis) was carried out,61 bands were amplified by 12 primers,53 bands were polymorphic,which accounted for 87.08% and each primer combination amplified 5.08 bands on average.All the 17 cultivars were divided into two groups.The results indicated that ISSR molecular markers had high efficiency and good repeatability,which was a useful tool for the analysis of the relationship among tea cultivars. |
| Keywords: | ISSR tea tree phylogenic analysis molecular marker |
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