| 产纤维素酶菌株的筛选及产酶条件的选择 |
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| 引用本文： | 林祥木,童金秀,陈汉清,庞杰.产纤维素酶菌株的筛选及产酶条件的选择[J].福建农林大学学报(自然科学版),2003,32(4):510-513. |
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| 作者姓名： | 林祥木 童金秀 陈汉清 庞杰 |
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| 作者单位： | 福建农林大学食品科技学院,福建,福州,35002 |
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| 基金项目： | 福建省科技厅重大科技项目(2003Y008)资助. |
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| 摘 要： | 采用摇床液体发酵试验,对18个菌株产纤维素酶进行滤纸酶活性、CMC酶活性、β-葡萄糖苷酶活性测定,筛选出1株产纤维素酶活性较高的菌株(C真3),并通过正交试验,确定该菌株的最优产酶条件.结果表明,最佳组合条件是液体发酵时间7d,摇床培养温度30℃,起始粗酶发酵培养基pH值5.5.
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| 关 键 词： | 纤维素酶 酶活性 液体发酵 正交试验 |
| 文章编号： | 1006-7817(2003)04-0510-04 |
| 修稿时间： | 2002年10月14 |
The isolation of strains producing cellulases and the conditions of enzyme production |
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| LIN Xiang-mu,TONG Jin-xiu,CHEN Han-qing,PANG Jie.The isolation of strains producing cellulases and the conditions of enzyme production[J].Journal of Fujian Agricultural and Forestry University,2003,32(4):510-513. |
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| Authors: | LIN Xiang-mu TONG Jin-xiu CHEN Han-qing PANG Jie |
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| Abstract: | The strain C_(fung3) was isolated from eighteen strains producing cellulase through liquid-state fermentation. The filter paper enzyme activity, CMC-enzyme activity and β-glucosidase activity of C_(fung3) were higher. Through orthogonal test, the optimal conditions of enzyme production were studied. The results showed that the optimal conditions were: 7 days~′ fermentation, 30 ℃, pH 5.5. |
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| Keywords: | cellulase enzyme activity liquid-state fermentation orthogonal experiment |
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