| 姬松茸多酚氧化酶的动力学特性 |
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| 引用本文： | 刘文聪.姬松茸多酚氧化酶的动力学特性[J].福建农林大学学报(自然科学版),2009,38(5). |
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| 作者姓名： | 刘文聪 |
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| 作者单位： | 福建农林大学食品科学技术研究所,福建,福州,350002 |
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| 摘 要： | 测定了姬松茸不同部位的多酚氧化酶(PPO)活性,研究了不同pH、温度、底物浓度和抑制剂对PPO活性的影响,并建立酶促褐变反应动力学方程.结果表明:在邻苯二酚为底物的条件下,姬松茸PPO的最适pH为6.4,最适温度为30℃,米氏常数(Km)为6.23×10-3mol.L-1,最大反应速度(Vmax)为1.42 mol.L-1.min-1;柠檬酸对PPO的抑制效果不明显,NaHSO和异V-Na对PPO均有较强的抑制作用,且有效抑制的含量分别为0.18%和0.21%.
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| 关 键 词： | 姬松茸 多酚氧化酶 动力学特性 |
Kinetics of polyphenol oxidase in Agaricus blazei |
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| LIU Wen-cong.Kinetics of polyphenol oxidase in Agaricus blazei[J].Journal of Fujian Agricultural and Forestry University,2009,38(5). |
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| Authors: | LIU Wen-cong |
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| Institution: | LIU Wen-cong(Institute of Food Science and Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | The polyphenol oxidase (PPO) activities in different parts of Agaricus blazei Murrill were determined, the effects of pH value, temperature, substrate concentration and enzyme inhibitors on PPO were studied, too. The results showed that the optimum pH value and optimum temperature for PPO were pH 6.4 and 30 ℃, respectively, the Michaelis constant (K_m) and V_(max) of PPO with pyrocatechol substrate were 6.23 × 10~(-3) mol · L~(-1) and 1.42 mol · L~(-1) · min~(-1) respectively using catechol as substrates, and the reaction kinetic equation was established. The inhibition of citric acid on PPO was not satisfactory, NaHSO_3 and sodium d-isoascorbate could inhibit effectively, and the effective concentrations were 0.18% and 0.21% , respectively. |
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| Keywords: | Agaricus blazei polyphenol oxidase kinetic characteristics |
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