| ISO-DALT双向电泳方法的优化与改进 |
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| 引用本文： | 王经源,陈舒奕,梁义元,林文雄.ISO-DALT双向电泳方法的优化与改进[J].福建农林大学学报(自然科学版),2006,35(2):187-190. |
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| 作者姓名： | 王经源 陈舒奕 梁义元 林文雄 |
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| 作者单位： | 1. 福建农林大学农业生态研究所,福建,福州,350002
2. 宁德出入境检验检疫局,福建,宁德,352100 |
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| 基金项目： | 中国科学院资助项目;福建省教育厅科研项目;福建省自然科学基金 |
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| 摘 要： | 通过对ISO-DALT双向电泳技术进行优化与改进,建立了一套适合于UV-B胁迫下水稻蛋白质组分析的双向电泳技术.用此方法对经低剂量UV-B辐射处理的水稻品种IR64的叶片蛋白进行双向电泳,可以分辨出1200-1300个蛋白(肽)点,等电点(pI)在3.5-9.3之间,相对分子质量在10-120 ku之间,分辨率较高,且重复性较好.
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| 关 键 词： | ISO-DALT 双向电泳 蛋白质组 水稻 |
| 文章编号： | 1671-5470(2006)02-0187-04 |
| 收稿时间： | 2005-06-22 |
| 修稿时间： | 2005-11-14 |
Improvement of ISO-DALT electrophoresis system |
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| WANG Jing-yuan,CHEN Shu-yi,LIANG Yi-yuan,LIN Wen-xiong.Improvement of ISO-DALT electrophoresis system[J].Journal of Fujian Agricultural and Forestry University,2006,35(2):187-190. |
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| Authors: | WANG Jing-yuan CHEN Shu-yi LIANG Yi-yuan LIN Wen-xiong |
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| Institution: | 1. Institute of Agro-ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Ningde Entry-Exit Inspect and Quarantine Bureau, Ningde, Fujian 352100, China |
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| Abstract: | This paper introduced an improved ISO-DALT electrophoresis system for Oryza sativa proteomic responding to UV-B radiation.The leaf proteins of IR64 radiated with UV-B were extracted and separated with 2-dimention electrophoresis.About 1200-1300 protein spots were identified on the 2D-gel.The pI of these proteins ranged from 3.5 to 9.3 with molecular weight distributing from 10 to 120 ku. |
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| Keywords: | ISO-DALT |
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