| mRNA差异显示技术研究中外猪种肉质性状差异表达基因 |
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| 引用本文： | 覃立立,童雄,徐建,张豪,李加琪,王翀.mRNA差异显示技术研究中外猪种肉质性状差异表达基因[J].华南农业大学学报,2011,32(4):82-85. |
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| 作者姓名： | 覃立立 童雄 徐建 张豪 李加琪 王翀 |
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| 作者单位： | 华南农业大学广东省农业动物基因组学与分子育种重点实验室,广东广州,510642 |
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| 基金项目： | 广东省自然科学基金重点项目(9251064201000005); 广东省科技计划项目(2008B020600009) |
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| 摘 要： | 利用mRNA差异显示技术对广东省地方品种蓝塘猪和引进品种大白猪达到经济成熟时的眼肌组织mRNA进行比较分析,并对差异显示条带同收产物进行PCR扩增,检测到与肉质性状相关的5条ESTs在2个猪种中存在表达差异,其中ESTsp7在大白猪种中表达量高,而ESTsp8、ESTsp9、ESTsp10和ESTdp11在蓝塘猪中表达...
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| 关 键 词： | 猪 mRNA差异显示技术 肉质性状 ESTs |
Study on the Different Expression of Meat Quality Candidate Genes Between the Local and Exotic Pig Breeds Using mRNA Differential Display Technology |
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| QIN Li-li,TONG Xiong,XU Jian,ZHANG Hao,LI Jia-qi and WANG Chong.Study on the Different Expression of Meat Quality Candidate Genes Between the Local and Exotic Pig Breeds Using mRNA Differential Display Technology[J].Journal of South China Agricultural University,2011,32(4):82-85. |
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| Authors: | QIN Li-li TONG Xiong XU Jian ZHANG Hao LI Jia-qi and WANG Chong |
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| Institution: | QIN Li-li,TONG Xiong,XU Jian,ZHANG Hao,LI Jia-qi,WANG Chong(Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | Comparative mRNA expression analysis of the ophthalmoplegia was made between Lantang(one of the Chinese indigenous pig breeds) and Large White using mRNA differential display technology.Differential expression bands were recycled and re-amplified.Different expressions of five ESTss which were related to the meat quality traits were detected in the two pig breeds.ESTsp7 was expressed higher whereas ESTsp8,ESTsp9,ESTsp10 and ESTsp11 were expressed lower in the Large White.Through the NCBI,it was found that ES... |
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| Keywords: | pig mRNA differential display technology meat quality traits ESTs |
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