| 黄瓜花叶病毒外壳蛋白基因转化辣椒的研究 |
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| 引用本文： | 吴丽君,胡开林,吴智明.黄瓜花叶病毒外壳蛋白基因转化辣椒的研究[J].华南农业大学学报,2007,28(2):69-72. |
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| 作者姓名： | 吴丽君 胡开林 吴智明 |
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| 作者单位： | 华南农业大学,园艺学院,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金
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广东省科技厅科技计划 |
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| 摘 要： | 以辣椒品种'B162'为试材,探讨了通过根癌农杆菌介导法将黄瓜花叶病毒外壳蛋白(CMV-CP)基因转化辣椒的适宜条件.结果表明,将辣椒带柄子叶进行预培养2 d后,用D600 nm为0.3的根癌农杆菌菌液浸泡7 min,再经过共培养2 d后转移到筛选分化培养基中进行培养,有利于获得辣椒抗性不定芽.通过对获得的10株抗性不定芽进行PCR检测,其中有2株扩增出目的条带,初步证明CMV-CP基因已经转入辣椒不定芽中.
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| 关 键 词： | 辣椒 根癌农杆菌介导 CMV-CP基因 不定芽 PCR 黄瓜花叶 病毒 外壳蛋白 基因转化 辣椒品种 研究 Cucumber Mosaic Virus Coat Protein Gene Capsicum annuum 检测 不定芽 抗性 分化培养基 筛选 转移 共培养 浸泡 菌液 根癌农杆菌 预培养 |
| 文章编号： | 1001-411X（2007）02-0069-04 |
| 修稿时间： | 2006-06-13 |
Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus |
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| WU Li-jun,HU Kai-lin,WU Zhi-ming.Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus[J].Journal of South China Agricultural University,2007,28(2):69-72. |
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| Authors: | WU Li-jun HU Kai-lin WU Zhi-ming |
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| Institution: | College of Horticulture ,South China Agric. Univ. , Guangzhou 510642,China |
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| Abstract: | Agrobacterium-mediated transformation of pepper 'B162'with coat protein gene of cucumber mosaic virus(CMV-CP) was studied.Results showed that the best transformation system was: cotyledons explants were precultured for 2 d,and then inoculated with Agrobacterium tumefaciens(D600 nm= 0.3) for 7 min,transferred onto selection differentiation medium after co-cultured for 2 d.10 green adventitious buds with Kan-resistance were screened by Polymerase chain reaction.Two of them were amplified with the band of the fragment of 740 bp.The result showed the CMV-CP gene had been incorporated into the plant genome successfully. |
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| Keywords: | Capsicum annuum Agrobacterium-mediated CMV-CP gene adventitious buds PCR |
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