3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析 Analysis of genetic information in <i>HA</i> genes and antigen specificities of three strains of H1N1 swine influenza virus期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析

引用本文：	秦锋,蔡孟楷,张伟东,张京,方博,黄俊明,卜德新,罗勇峰,马骏,付新亮,曹振鹏,张桂红.3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析[J].华南农业大学学报,2017,38(5):13-18.

作者姓名：	秦锋蔡孟楷张伟东张京方博黄俊明卜德新罗勇峰马骏付新亮曹振鹏张桂红

作者单位：	1. 广东省动物源性人兽共患病预防与控制重点实验室/国家生猪种业工程技术研究中心/华南农业大学兽医学院,广东广州,510642;2. 华南农业大学医院,广东广州,510642;3. 烟台市福山区动物卫生监督所,山东烟台,261400

基金项目：	国家重点研发计划（2016YFD0500707）；国家生猪产业技术体系专项（CARS-36）；广东省科技计划（2013B020202002）；广东省条财处项目（2012B060400015）；广东省高等学校优秀青年教师培养计划（YQ201530）

摘要：	【目的】分析3株属于欧亚类禽(SWSS1)、经典(SWL6)与Pdm09H1N1(Pdm091057)分支的猪流感病毒的HA基因遗传信息及抗原的特异性,为HA基因抗原表位的功能研究及流感防控奠定基础。【方法】以SWSS1株、SWL6株和Pdm091057株流感病毒为材料,比较分析3株H1N1亚型HA基因片段的遗传信息,制备全病毒灭活疫苗,各免疫3只雌性新西兰大白兔,采用血凝抑制(Hemagglutination inhibition,HI)反应试验检测抗体滴度。【结果】3株病毒的HA基因片段的氨基酸序列相似性为69.4%~89.1%,各分支的HA基因的抗原表位存在差异;3株病毒经过2次免疫后平均HI抗体滴度均能达1 280以上,且SWSS1的平均HI抗体滴度高达2 560。同时SWSS1与SWL6、Pdm091057这2株病毒均无血清学交叉反应,而SWL6与Pdm091057有较低的血清学交叉反应。【结论】3株猪流感病毒的HA基因片段抗原表位存在着差异,可能是3毒株之间血清学交叉反应较低的原因。3株病毒免疫原性均较好,可作为候选疫苗株。
关键词：	猪流感病毒 H1N1 遗传信息抗原表位血凝抑制抗体滴度 HA基因
收稿时间：	2016/10/19 0:00:00
Analysis of genetic information in HA genes and antigen specificities of three strains of H1N1 swine influenza virus

QIN Feng,CAI Mengkai,ZHANG Weidong,ZHANG Jing,FANG Bo,HUANG Junming,BU Dexin,LUO Yongfeng,MA Jun,FU Xinliang,CAO Zhenpeng and ZHANG Guihong.Analysis of genetic information in HA genes and antigen specificities of three strains of H1N1 swine influenza virus[J].Journal of South China Agricultural University,2017,38(5):13-18.

Authors:	QIN Feng CAI Mengkai ZHANG Weidong ZHANG Jing FANG Bo HUANG Junming BU Dexin LUO Yongfeng MA Jun FU Xinliang CAO Zhenpeng and ZHANG Guihong

Institution:	Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Hospital of South China Agricultural University, Guangzhou 510642, China,Animal Health Supervision of Fushan District, Yantai 261400, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China and Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University, Guangzhou 510642, China

Abstract:	Objective] To study the genetic information in HA genes and antigen specificities of SWSS1, SWL6 and Pdm091057 strains which respectively belongs to Eurasian avaian-like, classical and Pdm09H1N1 branches of H1N1 swine influenza viruses (SIV), and provide a basis for functional study on antigenic epitopes of HA gene as well as prevention and control of influenza virus.Method] We compared the genetic information in HA gene fragments of three H1N1 subtypes, SWSS1, SWL6 and Pdm091057. Inactivated SIV vaccines were prepared and each type of vaccine was used to immune three New Zealand white rabbit. The hemagglutination inhibition (HI) reaction was used to detect antibody titers.Result] The amino acid sequences of HA gene fragments had 69.4%–89.1% similarities among three SIV strains. There were variations in antigenic epitopes of HA genes of the three strains. The average HI antibody titers were all above 1 280 for three strains after the second immunization, and the average HI antibody titer for SWSS1 reached 2 560. SWSS1 had no serological cross reaction with SWL6 or Pdm091057 strain, while SWL6 and Pdm091057 strains had low degree of serological cross reaction.Conclusion] Three SIV strains have differences in antigenic epitopes of HA gene, which may be the reason for the low degree of serological cross reaction among strains. All three strains have good immunogenicity, and can be used as candidate vaccine strains.

Keywords:	swine influence virus H1N1 genetic information antigenic epitope hemagglutination inhibition antibody titer HA gene
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