| 乙烯和1-MCP对白姜花萜类香气及相关基因表达的影响 |
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| 引用本文： | 刘晓洲,范燕萍,余让才,岳跃冲,李昕悦,玉云祎.乙烯和1-MCP对白姜花萜类香气及相关基因表达的影响[J].华南农业大学学报,2018,39(4):68-72. |
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| 作者姓名： | 刘晓洲 范燕萍 余让才 岳跃冲 李昕悦 玉云祎 |
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| 作者单位： | 华南农业大学林学与风景园林学院;华南农业大学花卉研究中心 |
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| 基金项目： | 国家自然科学基金（31770738，313370694） |
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| 摘 要： | 【目的】研究乙烯和1-MCP对白姜花萜类香气物质合成释放的影响,探究相关基因的表达变化。【方法】以白姜花Hedychium coronarium为材料,分别用10μL·L~(–1)的乙烯和4μL·L~(–1)的乙烯抑制剂1-MCP处理8 h,随后采用顶空固相微萃取和气相色谱–质谱(GC-MS)技术进行挥发性香气物质的测定,所得到的离子图用NIST 08数据库进行定性及定量分析。通过荧光定量PCR分析相关基因的表达情况。【结果】乙烯处理后白姜花香气成分罗勒烯、沉香醇、别罗勒烯、石竹烯和法尼烯的挥发量显著上升,相关香气萜类合成酶基因HcTPS1、HcTPS3和HcTPS10的表达量分别上升了116%、182%和63%;而用1-MCP处理后,罗勒烯、别罗勒烯和法尼烯的挥发量显著下降,HcTPS1、HcTPS3和HcTPS10基因的表达量分别降低了45%、30%和30%。乙烯处理后,核转录因子HcEIL1-1和HcEIL1-2的表达量分别上升了51%和114%;1-MCP处理后,HcEIL1-1和HcEIL1-2表达量分别下降了30%和60%。【结论】外源乙烯可以提升白姜花萜类花香物质的释放量,白姜花乙烯信号通路中的核转录因子HcEIL1-1和HcEIL1-2与萜类花香合成酶基因的表达模式一致。
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| 关 键 词： | 白姜花 香气 乙烯 1-MCP 基因表达 萜类化合物 |
| 收稿时间： | 2018/1/19 0:00:00 |
Effect of ethylene and 1-MCP on the terpenoid fragrance of Hedychium coronarium and the expressions of related genes |
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| LIU Xiaozhou,FAN Yanping,YU Rangcai,YUE Yuechong,LI Xinyue and YU Yunyi.Effect of ethylene and 1-MCP on the terpenoid fragrance of Hedychium coronarium and the expressions of related genes[J].Journal of South China Agricultural University,2018,39(4):68-72. |
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| Authors: | LIU Xiaozhou FAN Yanping YU Rangcai YUE Yuechong LI Xinyue and YU Yunyi |
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| Institution: | College of Forestry and Landscape Archetecture, South China Agricutural University, Guangzhou 510642, China;Guangzhou Flower Research Center, South China Agricutural University, Guangzhou 510642, China,College of Forestry and Landscape Archetecture, South China Agricutural University, Guangzhou 510642, China;Guangzhou Flower Research Center, South China Agricutural University, Guangzhou 510642, China,Guangzhou Flower Research Center, South China Agricutural University, Guangzhou 510642, China,College of Forestry and Landscape Archetecture, South China Agricutural University, Guangzhou 510642, China;Guangzhou Flower Research Center, South China Agricutural University, Guangzhou 510642, China,College of Forestry and Landscape Archetecture, South China Agricutural University, Guangzhou 510642, China;Guangzhou Flower Research Center, South China Agricutural University, Guangzhou 510642, China and College of Forestry and Landscape Archetecture, South China Agricutural University, Guangzhou 510642, China;Guangzhou Flower Research Center, South China Agricutural University, Guangzhou 510642, China |
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| Abstract: | |
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| Keywords: | Hedychium coronarium fragrance ethylene 1-MCP gene expression terpenoid |
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