| 枯草芽孢杆菌BSF01菌剂制备及对高效氯氰菊酯的降解效果 |
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| 引用本文： | 周亮成,李运,李卓苗,林培炯,钟国华.枯草芽孢杆菌BSF01菌剂制备及对高效氯氰菊酯的降解效果[J].华南农业大学学报,2018,39(3):54-59. |
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| 作者姓名： | 周亮成 李运 李卓苗 林培炯 钟国华 |
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| 作者单位： | 华南农业大学农学院/农业部华南作物有害生物综合治理重点实验室 |
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| 基金项目： | 广东省科技计划项目（2015B020207003）；广东大学生科技创新培育专项（PDJH2016B0085） |
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| 摘 要： | 【目的】制备高效氯氰菊酯(β-CP)的降解菌菌剂。【方法】将前期分离驯化的β-CP高效降解菌株枯草芽孢杆菌Bacillus subtilis BSF01,通过各助剂成分及含量优化,获得菌剂最佳配方。采用室内模拟试验,检验该菌剂对土壤中β-CP的降解效果。【结果】试验明确了BSF01菌剂的最佳配方为:以w为30%的BSF01菌粉为主体,添加w为5%的十二烷基苯磺酸钠作为润湿剂、w为7%的阿拉伯树胶为分散剂、w为4%的磷酸钾为稳定剂和w为1%的糊精为保护剂,并补足载体高岭土至w为100%。该菌剂主要质量指标的检测结果表明,BSF01菌剂芽孢含量为6.50×10~(11) cfu·g~(-1),含水量(w)≤0.6%,细度≥99%,润湿时间为32.0 s,悬浮率为78.0%,符合国家标准。将BSF01菌剂稀释100、1 000及2 000倍后,分别投入受β-CP污染的田间土壤,5 d后,以100倍菌剂稀释液处理的土壤中β-CP降解率达到87.50%,有效降低了土壤中β-CP的残留水平。【结论】枯草芽孢杆菌BSF01菌剂可成为消除农田拟除虫菊酯类农药残留污染的高效安全生物制品。
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| 关 键 词： | 高效氯氰菊酯 枯草芽孢杆菌 生物降解 菌剂 农药 |
| 收稿时间： | 2017/10/9 0:00:00 |
Preparation of bacterial agent based on Bacillus subtilis BSF01 and its biodegradation effect on beta-cypermethrin |
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| ZHOU Liangcheng,LI Yun,LI Zhuomiao,LIN Peijiong and ZHONG Guohua.Preparation of bacterial agent based on Bacillus subtilis BSF01 and its biodegradation effect on beta-cypermethrin[J].Journal of South China Agricultural University,2018,39(3):54-59. |
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| Authors: | ZHOU Liangcheng LI Yun LI Zhuomiao LIN Peijiong and ZHONG Guohua |
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| Institution: | College of Agriculture, South China Agricultural University/Key Laboratory of Integrated Pest Management of Crop in South China, Ministry of Agriculture, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/Key Laboratory of Integrated Pest Management of Crop in South China, Ministry of Agriculture, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/Key Laboratory of Integrated Pest Management of Crop in South China, Ministry of Agriculture, Guangzhou 510642, China,College of Agriculture, South China Agricultural University/Key Laboratory of Integrated Pest Management of Crop in South China, Ministry of Agriculture, Guangzhou 510642, China and College of Agriculture, South China Agricultural University/Key Laboratory of Integrated Pest Management of Crop in South China, Ministry of Agriculture, Guangzhou 510642, China |
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| Abstract: | |
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| Keywords: | beta-cypermethrin Bacillus subtilis biodegradation bacterial agent pestiside |
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