| 茄子种质资源的ISSR遗传多样性分析 |
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| 引用本文： | 肖熙鸥,王勇,李冠男,曹必好,雷建军,陈国菊,陈清华.茄子种质资源的ISSR遗传多样性分析[J].华南农业大学学报,2012,33(3):296-300. |
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| 作者姓名： | 肖熙鸥 王勇 李冠男 曹必好 雷建军 陈国菊 陈清华 |
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| 作者单位： | 华南农业大学园艺学院,广东广州,510642 |
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| 基金项目： | 广东省自然科学基金，国家自然科学基金，广东省自然科学基金研究团队项目 |
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| 摘 要： | 利用ISSR分子标记分析茄子种质多样性,从100条引物中筛选出20条能够扩增出多态性条带稳定清晰的ISSR引物,对48份茄子材料的DNA进行扩增,共获得了186条谱带,其中多态性带153条(占所有谱带的82.2%).利用UPGMA法建立了48份茄子及其近缘野生种间的遗传关系聚类图.聚类结果表明,48份供试种质间的遗传相似系数分布在0.52～0.98之间,可将其分为5大类群.其中又将44份栽培品种分为5组,其品种间相似遗传系数在0.84～0.98之间,表明栽培品种的遗传背景比较窄.聚类结果与茄子的果形以及地理来源有一定的相关性,而与果色呈现不完全相关.
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| 关 键 词： | 茄子 ISSR 遗传多样性 相关分析 |
| 收稿时间： | 2011/10/17 0:00:00 |
ISSR Analysis of Genetic Diversity Among Cultivated Eggplant and Wild Relatives |
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| XIAO Xi-ou,WANG Yong,LI Guan-nan,CAO Bi-hao,LEI Jian-jun,CHEN Guo-ju and CHEN Qing-hua.ISSR Analysis of Genetic Diversity Among Cultivated Eggplant and Wild Relatives[J].Journal of South China Agricultural University,2012,33(3):296-300. |
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| Authors: | XIAO Xi-ou WANG Yong LI Guan-nan CAO Bi-hao LEI Jian-jun CHEN Guo-ju and CHEN Qing-hua |
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| Institution: | (College of Horticulture,South China Agricultural University,Guangzhou 501642,China) |
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| Abstract: | The genetic relationship and diversity of 44 eggplants, Solanum melongena L., germplasms and 4 wild relatives were analyzed by ISSR method .One hundred and fifty-three of 186 bands were amplified by 20 ISSR primers, about 82.2% being polymorphisms. Fourty-eight germplasms were clustered into 5 groups by genetic similar index from 0.52 to 0.98, and the wild species were different from eggplants. Also 44 eggplant cultivars had a high genetic similarity and a narrow genetic diversity. At the same time, the results showed that the cluster was related to the fruit shape of eggplants and origin areas, but not to the fruit colour. |
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| Keywords: | eggplant ISSR genetic diversity correlation analysis |
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