| 转PRSV复制酶基因T2代番木瓜植株的抗病性测定 |
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| 引用本文： | 阮小蕾,李华平,周国辉.转PRSV复制酶基因T2代番木瓜植株的抗病性测定[J].华南农业大学学报,2004,25(4):12-15. |
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| 作者姓名： | 阮小蕾 李华平 周国辉 |
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| 作者单位： | 华南农业大学,植物病毒研究室,广东,广州,510642 |
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| 基金项目： | 广东省重大科技项目(2002A208010202) |
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| 摘 要： | 转PRSV复制酶基因的番木瓜植株，其自交及杂交二代植株经PCR检测和Southem-blot杂交的结果表明：目的基因整合在番木瓜的染色体上．人工接种PRSV的Ya、Vb、Sm株系于分子检测阳性的7～8叶期植株，转基因植株均表现高抗．在定植田间的9个月中，转基因植株无一发病，而对照则全部发病．
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| 关 键 词： | 转PRSV复制酶基因 T2代番木瓜 植株 抗病性测定 |
| 文章编号： | 1001-411X(2004)04-0012-04 |
Evaluation of PRSV resistance of T2 transgenic papaya with replicase gene |
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| RUAN Xiao-lei,LI Hua-ping,ZHOU Guo-hui.Evaluation of PRSV resistance of T2 transgenic papaya with replicase gene[J].Journal of South China Agricultural University,2004,25(4):12-15. |
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| Authors: | RUAN Xiao-lei LI Hua-ping ZHOU Guo-hui |
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| Abstract: | Results from PCR and southern-blot analysis conformed that the target gene was integrated into the genome of T_2 transgenic papaya (Carica papaya) plants with replicase gene of papaya ringspot virus (PRSV). These transgenic papaya seedlings in 7-8 leaf stages were mechanically inoculated respectively with three severe strains of PRSV in South China. They showed a high resistant to all strains of PRSV. By transplanting these seedlings to field, no any symptom appeared in these transgenic plants during 9 months. On the contrary, the control plants showed very severe symptoms. |
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| Keywords: | Carica papaya replicase gene resistance |
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