| 昆明小鼠单细胞胚胎体外培养的研究 |
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| 引用本文： | 张守全,孙拓.昆明小鼠单细胞胚胎体外培养的研究[J].华南农业大学学报,1993,14(1):10-15. |
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| 作者姓名： | 张守全 孙拓 |
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| 作者单位： | 华南农业大学畜牧系
(张守全)，北京农业大学畜牧系(孙拓) |
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| 摘 要： | 本试验研究了葡萄糖、EDTA和小鼠血清对昆明小鼠单细胞胚体外发育的影响.结果发现:不含葡萄糖HECM-1组桑椹率为40.05％,对照组为8.14％;含EDTA的CZB组桑椹率为61.18％,对照组为47.44％;小鼠血清对昆明小鼠单细胞胚体外发育无促进作用。可见,葡萄糖抑制昆明小鼠单细胞胚胎体外发育;在不含葡萄糖的条件下,EDTA支持小鼠早期胚胎体外发育;无论有无葡萄糖小鼠血清对小鼠早期胚体外发育无促进作用。
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| 关 键 词： | 小鼠 胚胎 体外培养 细胞 |
STUDY ON CULTURING KUMING MOUSE 1-CELL EMBRYOS IN VITRO |
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| Zhang Shouquan Sun Tuo.STUDY ON CULTURING KUMING MOUSE 1-CELL EMBRYOS IN VITRO[J].Journal of South China Agricultural University,1993,14(1):10-15. |
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| Authors: | Zhang Shouquan Sun Tuo |
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| Institution: | Zhang Shouquan Sun Tuo(Dept of animal science) (Dept. of animal scienceBeijing Agricultural Univeratity) |
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| Abstract: | This study was dealt with the effects of glucose and mouse serum and EDTA on Kuming mouse 1-cell embryo development in vitro. The results show that the precentage of morula embryos cultured in glucose free HECM-1 to 40. 05%, and the control only 8.14%, cultured in CZB containing 0.11mM EDTA is 61. 18%, the control 47. 44%. In conclusion, the presence of glucose in culture medium is detrimental to Kuming mouse 1-cell embryo development. EDTA supports mouse embryo developement in vitro in glucose -free medium also. Glucose lacked or not, mouse serum doesn't affected in vitro mouse embrvo development. |
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| Keywords: | Kuming mouse 1-cell embryo Glucose EDTA Mouse serum Culture In vitro |
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