研究植物胚囊中微管的PEG切片法的改进

经改良的PEG切片法，操作简单易行，观察结果清晰。水稻子房经固定、脱水、包埋、切片、真空粘片法粘片、荧光抗体标记等处理后，在激光共聚焦扫描显微镜下观察胚囊中的微管并拍照。传统粘片法（如多聚赖氨酸粘片法）中的粘片剂阻碍了抗体与微管的结合（致使微管不易被标记出），本文提出的真空粘片法从根本上解决了这一问题，这一新的粘片方法的采用，是使PEG切片法的观察效果获得了较大改进的主要原因，真空粘片法的一个最主要特点是不需要粘片剂，该法解决了由粘片剂所引起的一系列问题，适用于既需粘片、而粘片剂又影响以后操作的情况，有较大的推广价值。

Improved Method of PEG Embedding Section Being Used in Investigating Microtubules in Plant Embryo Sac

Improved PEG embedded section method was described. The method made it easy to operate and the results were obviously clearer. Ovaries of rice were conducted by fixing, dehydrating, embedding by PEG in small centrifuge tube, cutting section by razor, vacuum gluing section, labeling by fluorescence antibody, and then microtubules in embryo sac were observed and photographed by confocal laser scanning microscopy. The former gluing section methods (such as poly lysine gluing section method) blocked severely the conjuncting between microtubules and antibodies, vacuum gluing section method being founded by us resolved the problem, the new gluing section method was the main reason leading to improvement of results. One most obvious feature of vacuum gluing section method was without sticker. The method resolved all problems caused by sticker, and the method was suitable for the situation that needed gluing section but sticker blocked the later conducting,and the method was possible to be widely used in other researches.