山梨醇对籼稻愈伤组织培养的效应

以6个籼稻品种为供试材料,研究结果肯定了培养基中附加成分山梨醇在愈伤组织继代培养中,具有促进愈伤组织生长及提高其分化潜力的效果;籼稻亚种中的不同品种,愈伤组织的生长及再生植株能力,在继代培养的后期表现出明显的品种特异性;分化培养基中加入不同浓度配比激素,对诱导器官分化的效果有明显差别,激动素(KT)与萘乙酸(NAA)的比例为5:1有利于取得根、芽生长的同步性。

EFFECTS OF SORBITOL ON INDICA RICE CALLUS CULTURE

Using six varieties of indica rice as experimental materials, the study results indicated that sorbitol added to Ls medium was able to promote the growing of callus and improve its differentiation in serial subcultures. For different varieties of indica rice the growing capacity of callus and plant regeneration displayed evident varietal specificities in later stages of subcultures. After adding phytohormone with different concentrations into the differentiating medium, there were obvious disparities in differentiation effects on the organ indicated.Synchronized growing of root and bud was favoured when-kinetin (KT)and naphthalene acetic acid (NAA) were present in the ratio of 5 : 1.