2个低直链淀粉含量籼稻突变体的遗传分析

对2个空间诱变低直链淀粉含量籼稻突变体XLA-1和XLA-2进行了遗传分析和分子生物学研究.结果表明:XLA-1低直链淀粉遗传特性受2对隐性基因控制,这2对基因具有连锁关系和互补作用,任何1对基因隐性纯合都将导致直链淀粉含量降低;XLA-2低直链淀粉遗传特性受1对隐性主效基因控制,该基因可能为Wx的等位基因,同时受微效基因的修饰.XLA-1和XLA-2蜡质基因(CT)n微卫星多态性与糯稻相同,但其直链淀粉质量分数分别为14.42%和11.59%,说明除Wx基因外确实还有其他影响直链淀粉含量的遗传因素,这与遗传分析结果相符.

Genetic Analysis of Low Amylose Content Trait in Two Oryza indica Mutants

Series of experiments of genetic analysis were carried out in this paper.The main results obtained as follows:(1) The low amylose content trait in XLA-1 was controlled by two recessive linked genes.The two genes had complementary interaction and any homozygous gene can reduce the amylase content.In these two genes,one was possibly a multiple allele to Wx gene and another gene maybe a new mutant gene.(2) One major recessive gene and several minor genes controlled the low amylose content trait in XLA-2.This gene maybe a multiple allele to Wx gene.(3) The genomic DNA polymorphism analysis with the(CT) n microsatellite marker 484/485 of Wx gene indicated that the genomic DNA polymorphism had no difference among XLA-1,XLA-2 and Jingxiangnuo(glutinous rice),but the amylase content of XLA-1,XLA-2 were 14.42% and 11.59%,respectively.The results showed that there were other genetic factors which could affect the amylase content.The result coincided with the genetic analysis.