| 西川红景天nrDNA ITS序列初步研究(英文) |
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| 引用本文： | 高庆波,张得钧,段义忠,张发起,陈世龙.西川红景天nrDNA ITS序列初步研究(英文)[J].农业科学与技术,2008,9(5):63-65. |
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| 作者姓名： | 高庆波 张得钧 段义忠 张发起 陈世龙 |
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| 作者单位： | 中国科学院高原生物适应与进化重点实验室中国科学院西北高原生物研究所;中国科学院研究生院 |
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| 基金项目： | 国家自然科学基金
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国家重点基础研究发展计划(973计划)
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瑞典国家自然科学基金
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| 摘 要： | Objective] The study aimed to analyze the ITS sequences of nrDNA from Rhodiola alisa and investigate the difference of evolution rate between nrDNA and trnS-trnG and rpl20-rps12 sequences of cpDNA(chloroplast DNA).Method]Total DNA was extracted from silica-dried leaves of R.alsia by using modified CTAB method.With the extracted DNA sample as template,nrDNA ITS region was amplified,then purified and sequenced.In addition,the yielded ITS sequences were also compared with the known trnS-trnG and rpl20-rps12 sequences of cpDNA from R.alsia.Result]The ITS sequence of nrDNA from R.alsia was 701 bp in length,of which 13 variable sites were found with a percentage of 1.85%.Of the 13 variable sites,8 were caused by point mutations,5 were the results of insertions or deletions.The(A+T)content and(G+C)content were 46.9% and 53.1%,respectively.The nucleotide diversity(π)was 0.004 27.Conclusion]The ITS region of nrDNA from R.alsia was more conservative and evolved more slowly than the trnS-trnG and rpl20-rps12 sequences of its cpDNA.
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| 关 键 词： | Rhodiola alsia ITS sequence trnS-trnG sequence rpl20-rps12 sequence |
Preliminary Study on the ITS Sequences of nrDNA from Rhodiola alsia |
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| GAO Qing-bo,ZHANG De-jun,DUAN Yi-zhong,ZHANG Fa-qi,CHEN Shi-long.Preliminary Study on the ITS Sequences of nrDNA from Rhodiola alsia[J].Agricultural Science ＆ Technology,2008,9(5):63-65. |
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| Authors: | GAO Qing-bo ZHANG De-jun DUAN Yi-zhong ZHANG Fa-qi CHEN Shi-long |
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| Institution: | 1.Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001;2.Graduate University of the Chinese Academy of Sciences,Beijing 100039 |
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| Abstract: | Objective] The study aimed to analyze the ITS sequences of nrDNA from Rhodiola alisa and investigate the difference of evolution rate be-tween nrDNA and trnS-trnG and rp/20-rps12 sequences of cpDNA (chloroplast DNA). Method] Total DNA was extracted from silica-dried leaves of R. alsia by using modified CTAB method. With the extracted DNA sample as template, nrDNA ITS region was amplified, then purified and sequenced. In addition, the yielded ITS sequences were also compared with the known trnS-trnG and rpt20-rps12 sequences of cpDNA from R. alsia. Result]The ITS sequence of nrDNA from R. alsia was 701 bp in length, of which 13 variable sites were found with a percentage of 1.85%. Of the 13 variable sites, 8 were caused by point mutations, 5 were the results of insertions or deletions. The (A+T) content and (G+C) content were 46.9% and 53.1%, respectively. The nucleotide diversity (π) was 0.004 27. Conclusion] The ITS region of nrDNA from R. alsia was more conservative and evolved more slowly than the traS-trnG and rp/20-rps12 sequences of its cpDNA. |
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| Keywords: | Rhodiola alsia ITS sequence trnS-trnG sequence rpt20-rps12 sequence |
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