软枣猕猴桃对低温胁迫的生理响应

目的]探明软枣猕猴桃对低温胁迫生理变化规律,为引种、抗寒品种选育提供理论依据.方法]以野生软枣猕猴桃幼苗为试材,通过4℃低温处理,以25℃(常温)为对照,测定软枣猕猴桃叶绿素含量、可溶性糖、可溶性蛋白、脯氨酸、丙二醛(MDA)的含量以及抗氧化酶(SOD、CAT、POD)的活性变化.结果]随着低温胁迫时间的延长,幼苗叶片叶绿素含量呈现先增后减趋势,MDA含量增加;可溶性糖含量上升,可溶性蛋白、脯氨酸含量呈先增后减趋势;SOD、POD活性均下降,CAT活性先升后降.结论]综合各项指标表明,叶片在低温胁迫过程中,叶绿素含量降低,膜脂过氧化反应加剧,MDA积累,可溶性糖、可溶性蛋白、脯氨酸等渗透调节物质以及抗氧化酶起到了不同程度的保护作用.

Physiological Responses of Actinidia arguta （Seib.et.Zucc.) to Low Temperature Stress

Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta (Seib.et.Zucc.),and provide a theoretical basis for the introduction and breeding of cold resistant varieties.Method] A.arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chlorophyll,water soluble sugar,water soluble protein,free proline,MDA,and antioxidant enzymes (POD activity,SOD activity,CAT activity) with the treatment at 25℃ as the control.Results] The results indicated that with the prolonging of stress time,the content of chlorophyll was first increased and then decreased,and the content of MDA was increased;soluble sugar content of seedling leaf decreased gradually,while the soluble protein and proline content of seedling leaves were first increased and then decreased;the activities of SOD and POD were displayed a decline trend,meanwhile the activities of CAT was first increased and then decreased.Conclusion] The various indicators showed that under low temperature stress,the chlorophyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation,and soluble sugar,soluble protein,free proline and antioxidant enzymes play a protective role in different degrees.