鸭坦布苏病毒对雏鸭血清生化指标、细胞因子和病毒复制情况的影响(英文)

为了解鸭坦布苏病毒(DTMUV)的致病性,试验以1×10~4EID_(50)的剂量DTMUV肌肉注射5日龄樱桃谷雏鸭,采用生化指标检测试剂盒对血清样品进行生化指标检测,采用荧光定量PCR方法检测排毒情况、组织载毒量和细胞因子的变化情况。结果表明,DTMUV对肝脏、肾脏、心脏和肌肉组织都有严重损伤;DTMUV可以在肝脏、脾脏、肺脏、脑中增殖,并于攻毒后第5天在肝脏和脑中达到高峰,于攻毒后第9天后在肺脏和脾脏中达到高峰,且脑、肝脏、脾脏中病毒含量最高;攻毒后第1天DTMUV就可引起脾脏中γ干扰素(IFN γ)、α干扰素(IFN α)、白细胞介素6(IL-6)、β干扰素(IFN β)、白细胞介素1β(IL-1β)、Toll样受体7(TLR-7)、白细胞介素2(IL-2)、主要组织相容性复合体Ⅰ型(MHC-Ⅰ)、主要组织相容性复合体Ⅱ型(MHC-Ⅱ)细胞因子过渡表达,在脑中第1,2,3 d均引起促炎性细胞因子IL-6、IL-2的过度应答。

Effects of Duck Tembusu Virus on Serum Biochemical Indexes,Cytokines and Viral Replication of Ducklings

In order to understand the pathogenicity of duck Tembusu virus (DTMUV), it was injected into muscle of 5-d-old Cherry Valley ducklings according to the dosage of 1×104 EID50. Then, the biochemical indexes of duckling serum samples were determined by kits, and the changes in detoxification, tissue viral load and cytokines were detected by using fluorescence quantitative PCR. The results showed that DTMUV had serious damage to the liver, kidney, heart and muscle of ducklings; DTMUV could proliferate in the liver, spleen, lung and brain; the virus levels in the liver and brain reached the peaks on day 5 after the inoculation and those in the lung and spleen reached the peaks on day 9; the virus content was highest in the brain, liver and spleen; and DTMUV induced the overexpression of IFN-γ, IFN-α, IL-6, IFN-β, IL-1β, TLR-7,IL-2, major histocompatibility complex type I (MHC-I) andmajor histocompatibility complex type II (MHC-II) in the spleen on day 1 and the overexpression of IL-6 and IL-2 in the brain on days 1, 2 and 3.