| 一种有效提取无核荔枝幼胚总RNA的方法 |
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| 引用本文： | 刘兴地,郑学勤.一种有效提取无核荔枝幼胚总RNA的方法[J].农业科学与技术,2008,9(1):29-31. |
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| 作者姓名： | 刘兴地 郑学勤 |
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| 作者单位： | [1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]海南大学农学院,海南儋州571737 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 笔者采用改良CTAB法从富含多酚的无核荔枝幼胚中提取总RNA，并用甲醛变性琼脂糖凝胶电泳和紫外分光光度计对所提RNA质量进行检测，旨在为多糖、多酚含量较高的植物材料RNA的提取提供一种有效方法。
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| 关 键 词： | 无核荔枝 幼胚 RNA 提取方法 |
An Effective Method for Extracting Total RNA from Young Embryo of Seedless Litchi |
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| LIU Xing-di,ZHENG Xue-qin.An Effective Method for Extracting Total RNA from Young Embryo of Seedless Litchi[J].Agricultural Science ＆ Technology,2008,9(1):29-31. |
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| Authors: | LIU Xing-di ZHENG Xue-qin |
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| Institution: | LIU Xing-di, ZHENG Xue-qin (1.Institute of Tropical Bioscience and Biotechnology CATAS,Haikou 571101;2.Agricultural College of Hainan University, Danzhou 571737) |
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| Abstract: | A total RNA extraction method for young embryo of seedless litchi was introduced.CRAB,Phenol(saturated with water),chloroform,Guanidine isothiecyanate were used as main extraction reagents.Polyphenolic compounds were removed effectively by added PVP into the extraction buffer solution.RNA was purified intensively by phenol,chloroform extraction,and ethanol deposition after deposited by LiCl.Both the results of formaldehyde denatured agarose gel electrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA.So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA. |
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| Keywords: | Seedless litchi Young embryo RNA extraction |
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