棉花开花前不同天数胚珠的采集标准检测

目的]检测棉花开花前不同天数胚珠的采集标准,以期更清楚地研究棉花开花前后胚珠中基因的表达变化。方法]采用花苞长、花苞宽、萼片长和萼片宽4种不同的参数来标准化陆地棉新陆早13号开花前不同天数棉花胚珠的特征。结果]只有花苞长随花期的临近逐渐增长,而其他参数均没有明显变化。因此,选择花苞长为检测开花前不同天数胚珠的标准。统计分析表明,确定以1.0～1.2cm长的花苞为标准采集开花前5d的胚珠,以1.3～1.5cm长的花苞为标准采集开花前3d的胚珠,以1.7cm以上的花苞为标准采集开花前1d的胚珠。结论]该研究结果为后期试验中开花前胚珠样品的采集提供参考数据,也为更精确检测开花前后胚珠中基因表达的变化奠定基础。

Determination of Collection Standard of Cotton Ovlues on Different Days before Flowering

Objective]The aim was to determine the collection standard of cotton ovules on different days before flowering,and then to more clearly study the changes in gene expression of cotton ovules before and after blooming.Method]The standard to distinguish ovules in Xinluzao 13 on different days before flowering was defined by the length and width of alabasterum and sepal.Result]The alabasterum length increased gradually with approaching of flowering time,while other parameters did not show the same tendency.Therefore,the alabasterum length was chosen as the standard to determine the ovules on different days before flowering.The results of statistics analysis revealed that the alabstrums with length of 1.0-1.2,1.3-1.5 cm and more than 1.7 cm respectively were determined as the standards for ovules collection on 5,3 and 1 d before flowering.Conclusion]This study will provide not only the reference data for the collection of ovules sample in the following experiment,but also the basis for more precisely detecting the changes in gene expression of ovules before and after flowering.