| 寿光黑鸡成纤维细胞的体外培养与冷冻保存(英文) |
| |
| 引用本文： | 王娟,于媛,王跃嗣,马云,焦飞.寿光黑鸡成纤维细胞的体外培养与冷冻保存(英文)[J].农业科学与技术,2008,9(6):136-141. |
| |
| 作者姓名： | 王娟 于媛 王跃嗣 马云 焦飞 |
| |
| 作者单位： | 王娟,王跃嗣,WANG Juan,WANG Yue-si(滨州医学院药学院细胞工程教研室,山东烟台);于媛,焦飞,YU Yuan,JIAO Fei(滨州医学院基础学院生物化学教研室,山东烟台,264003);马云,MA Yun(滨州医学院基础学院病理教研室,山东烟台,264003) |
| |
| 基金项目： | 国家自然科学基金，山东省教育厅基金 |
| |
| 摘 要： | Objective] The aim of this study was to establish the in vitro culture system of chicken fibroblasts.Method] Tissue explant method and enzymatic digestion method were used to separate and culture chicken skin fibroblasts respectively.The rate of cell growth,cryopreservation and recovery were compared.Result] The primary chicken fibroblasts prepared by enzymatic digestion grew faster and converged together to form monolayer on 5 d post preparation;the passage cells prepared by these 2 methods grew at similar speed and formed monolayer within 2-3 d;homogeneous fibroblasts could be obtained by trypsin digestion and repeated attachment for 3-4 passages;there were 75%-80% of cells survived after cryopreservation and recovery;the growth curves of embryonic fibroblasts and skin fibroblasts were all normal and the two kind of cells still retained the normal number of chromosomes even at the twelfth passage.Conclusion] The feeder layer cells needed for establishing ES cell lines could be obtained by culturing chicken fibroblasts through both tissue explant method and enzymatic digestion method.This study provided a basis for the successful establishment of ES cell lines.
|
| 关 键 词： | Skin Embryonic Fibroblasts Culture in vitro Cryopreservation |
Culture in vitro and Cryopreservation of Shouguang Black Chicken Fibroblasts |
| |
| WANG Juan,YU Yuan,WANG Yue-si,MA Yun,JIAO Fei.Culture in vitro and Cryopreservation of Shouguang Black Chicken Fibroblasts[J].Agricultural Science ＆ Technology,2008,9(6):136-141. |
| |
| Authors: | WANG Juan YU Yuan WANG Yue-si MA Yun JIAO Fei |
| |
| Institution: | 1.Department of Cell Engineering,College of Pharmaceutical Sciences,Binzhou Medical University,Yantai 264003;2.Department of Biochemistry,College of Basic Medical Sciences,Binzhou Medical University,Yantai 264003;3.Department of Pathology,College of Basic Medical Sciences,Binzhou Medical University,Yantai 264003 |
| |
| Abstract: | Objective] The aim of this study was to establish the in vitro culture system of chicken fibroblasts. Method] Tissue explant method and enzymatic digestion method were used to separate and culture chicken skin fibroblasts respectively. The rate of cell growth, cryopreservation and recovery were compared. Result] The primary chicken fibroblasts prepared by enzymatic digestion grew faster and converged together to form monolayer on 5 d post preparation; the passage cells prepared by these 2 methods grew at similar speed and formed monolayer within 2-3 d; homogeneous fibroblasts could be obtained by trypsin digestion and repeated attachment for 3-4 passages; there were 75%-80% of cells survived after cryopreservation and recovery; the growth curves of embryonic fibroblasts and skin fibroblasts were all normal and the two kind of cells still retained the normal number of chromosomes even at the twelfth passage. Conclusion] The feeder layer cells needed for establishing ES cell lines could be obtained by culturing chicken fibroblasts through both tissue explant method and enzymatic digestion method. This study provided a basis for the successful establishment of ES cell lines. |
| |
| Keywords: | Skin Embryonic Fibroblasts Culture in vitro Cryopreservation |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
