锰胁迫对大豆幼苗POD活性及其同工酶的影响

目的]在分子水平上探讨不同品种大豆在锰毒害下的一些遗传特性和基因差异的本质原因。研究不同锰浓度（0～120mg/L）对大豆幼苗水栽培,苗龄10d的根系、茎部以及叶片的过氧化物酶（POD）活性的影响以及其同工酶谱的变化。结果]随锰浓度的提高,大豆幼苗的根系、茎部以及叶片的过氧化物酶（POD）活性均表现为先上升后下降的变化趋势。不同器官POD同工酶酶谱对锰胁迫的反应存在一定差异,其中根系同工酶的酶带数和活性变化较大,茎部与叶片的同工酶的酶带数和活性较稳定。结论]大豆幼苗不同器官的同工酶酶谱的变化与其对锰胁迫的抗性有关。

Effects of Manganese Stress on POD Activities and Isozymes of Soybean

Objective]The aim was to discuss the internal reason of some inherited characteristics and genetic differences of different varieties of poisoned soybeans in molecule level.Method]Soybeans were cultivated by water culture and treated for 10 days under natural light conditions.The influence of increasing concentration of manganese(0-120 mg/L) on the POD activities and isoenzyme of roots stems and leaves of soybean were studied.Result]Results showed that the POD activities in roots,stems and leaves firstly increased and then decreased with increasing concentration of manganese.There were differences in the response of POD isoenzyme zymograms to manganese stress in different organs,the POD isoenzyme activity and quantities of soybean roots changed apparently when the manganese concentration increased,while the POD isoenzyme activity and quantities of soybean stems and leaves kept stable.Conclusion]The isoenzyme zymograms changes of different organs of soybean were related to the resistance of soybean to manganese stress.