桑枝食用菌对1-脱氧野尻霉素的富集作用

目的]探讨食用菌对桑枝中1-脱氧野尻霉素（1-deoxynojimycin,DNJ）的富集作用。方法]利用反相高效液相色谱法,研究耳菇、香菇、金针菇、鸡腿菇、银耳、姬菇、黑木耳、灵芝、猴头菇9种食用菌对桑枝中1-脱氧野尻霉素（1-deoxyno jimycin,DNJ）的富集能力。结果]供试食用菌种均不能自我合成DNJ,但黑木耳、灵芝和猴头菇对DNJ有富集作用,而且其菌盖的富集能力远远高于菌柄。随着培养料中桑枝屑比例的增加,灵芝和猴头菇菌盖中DNJ含量也随之增加,在50%桑枝屑时达到最大,分别为0.0704%和0.0472%,再增加桑枝屑比例,则其菌盖中DNJ含量下降,当桑枝屑比例为80%时,其菌盖中DNJ含量分别降为0.0474%和0.0327%。结论]该研究可为开发具有降血糖功效的桑枝食用菌提供理论依据和参考。

Enrichment of Edible Fungi to I-deoxynojirimycin in Mulberry Branches

Objective] This research aimed to explore the enrichment of edible fungi to the DNJ in mulberry branches.Method] Reversed phase high performance liquid chromatography(RP-HPLC) method was used to study the enrichment of 9 varieties of edible fungi to the I-deoxynojirimycin(DNJ) in mulberry branches.Result] The test edible fungi all could not synthesize DNJ,but Auricularia auricular,Ganoderma lucidum and Hericium erinaceus had enrichment capability to DNJ,and the enrichment was higher in pileus than in stipe.With the increasing proportion of mulberry sawdust in cultivation materials,the contents of DNJ in Ganoderma lucidum and Hericium erinaceus had increased;when the proportion of mulberry sawdust had achieved 50%,the contents of DNJ in the pileus of Hericium erinaceus and Ganoderma lucidum reached the maximum,which were 0.070 4% and 0.047 2%,respectively;afterwards,the contents of DNJ in the pileus were reduced with the increasing proportion of mulberry sawdust;when the proportion of mulberry sawdust had achieved 80%,the contents of DNJ in the pileus of Hericium erinaceus and Ganoderma lucidum had decreased to 0.047 4% and 0.032 7%,respectively.Conclusion] This study provided theoretical basis and reference for the development of edible fungi in mulberry branches with hypoglycemic effects.