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利用微卫星标记分析湖羊GDF8区域的遗传多样性
引用本文:吴文忠,钱建共,陈玲,孙伟.利用微卫星标记分析湖羊GDF8区域的遗传多样性[J].农业科学与技术,2009,10(4):68-71.
作者姓名:吴文忠  钱建共  陈玲  孙伟
作者单位:吴文忠,钱建共,陈玲(江苏省苏州市畜牧兽医站,江苏苏州,215128);孙伟(扬州大学动物科学与技术学院,江苏扬州,225009) 
基金项目:the State Scientific Basic Research Platform Program,China Postdoctoral Science Foundation,Natural Science Foundation of Jiangsu Province of China,the National high-tech R&D program(863program),Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period,Support Foundation of China during the 11th Five-Year Plan Period,Basic Natural Science Foundation for Colleges and Universities in Jiangsu Province,Jiangsu Government Scholarship for Overseas Studies Project,Qing Lan Project of Colleges and Universities Jiangsu Province;and the New Century Talent Project of Yangzhou University in China.国家科技基础条件平台项目,中国博士后科学基金,江苏省自然科学基金,国家高技术研究与发展计划(863),国家"十一五"科技支撑,"十一五"国家科技支撑计划重点项目,江苏省教育厅基金项目,江苏省政府留学奖学金,江苏省高校"青蓝工程"优秀青年骨干教师培养基金和扬州大学"新世纪人才工程"优秀骨干教师培养基金 
摘    要:1材料与方法 1.1试验材料与DNA提取2007年,在江苏省苏州市种羊场随机选取成年羊88只,采取耳组织提取DNA,-20℃保存。并记录湖羊的初生重、断奶重和6月龄重。

关 键 词:微卫星标记分析  遗传多样性  湖羊  DNA提取  试验材料  成年羊  种羊场  苏州市

Genetic Polymorphism in GDF8 Region of HU Sheep Based on Microsatellite DNA
WU Wen-zhong,QIAN Jian-gong,CHEN Ling,SUN Wei.Genetic Polymorphism in GDF8 Region of HU Sheep Based on Microsatellite DNA[J].Agricultural Science & Technology,2009,10(4):68-71.
Authors:WU Wen-zhong  QIAN Jian-gong  CHEN Ling  SUN Wei
Institution:1, Animal Science & Veterinary Medicine Bureau of Suzhou City, Suzhou 215128; 2, Animal Science and Technology College of Yangzhou University, Yangzhou 225009)
Abstract: Objective ] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 of sheep that may be correlated with growth performance were chosen to detect the molecular genetics foundation of growth performance of Hu sheep. Result] Four microsatellite loci detected were high in heterozygosity, more in effective alleles number and rich in polymorphic information, all the three indices passed through the high polymorphic level (PIC 〉0.5). Conclusion ] The four microsatellite loci detected could be used to estimate the genetic polymorphism of growth performance of Hu sheep.
Keywords:Hu sheep  GDF8  Microsatellite DNA  Growth performance
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