| 抗犬瘟热病毒荧光标记单抗的制备和初步鉴定 |
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| 引用本文： | 苏建青,褚秀玲,杨松涛,夏成柱,岳妙姝.抗犬瘟热病毒荧光标记单抗的制备和初步鉴定[J].农业科学与技术,2009,10(1):115-118,144. |
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| 作者姓名： | 苏建青 褚秀玲 杨松涛 夏成柱 岳妙姝 |
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| 作者单位： | 苏建青,褚秀玲,SU Jian-qing,CHU Xiu-ling(聊城大学农学院,山东聊城,252059);杨松涛,夏咸柱,YANG Song-tao,XIA Xian-zhu(中国人民解放军军事医学科学院军事兽医研究所,吉林长春,130062);岳妙姝,YUE Miao-shu(长春理工大学生命科学技术学院,吉林长春,130062)
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| 基金项目： | 总后科技攻关资助项目 |
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| 摘 要： | 1材料与方法
1．1材料 1．1．1病毒与细胞。犬瘟热病毒（CDV）、狂犬病毒（RV）、犬细小病毒（CPV）、犬副流感病毒（CPIV）、犬腺病毒（CAV），非洲绿猴肾细胞（Vero）、犬肾细胞（MDCK）、猫肾细胞（F81），犬瘟热病毒单克隆抗体CE3由军事医学科学院军事兽医研究所犬病研究中心提供。
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| 关 键 词： | 犬瘟热病毒 荧光标记 非洲绿猴肾细胞 鉴定 制备 单抗 犬副流感病毒 狂犬病毒 |
Preparation and Preliminary Identification of Fluorescein Labeled Monoclonal Antibody against Canine Distemper Virus |
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| SU Jian-qing,CHU Xiu-ling,YANG Song-tao,XIA Xian-zhu,YUE Miao-shu.Preparation and Preliminary Identification of Fluorescein Labeled Monoclonal Antibody against Canine Distemper Virus[J].Agricultural Science ＆ Technology,2009,10(1):115-118,144. |
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| Authors: | SU Jian-qing CHU Xiu-ling YANG Song-tao XIA Xian-zhu YUE Miao-shu |
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| Institution: | 1. College of Agriculture, Liaocheng University, Liaocheng 252059; 2. Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Changchun 130062; 3. College of Life Science and Technology, Changchun University of Science and Technology, Changchun 130062) |
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| Abstract: | Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper (CD) with FITC-conjugated monoclonal antibodies (FITC-McAb). Method] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofluorescence assay. Result] The absorption test, blocking test and specificity test showed that the labeled antibody had high specificity and sensitivity, but didnt have cross reaction with canine parvovirus (CPV), canine parainfluenza virus (CPIV), canine adenovirus (CAV) and rabies virus (RV). The optimal working concentration was 1∶80. The positive rate of clinical suspected samples was 48%. Conclusion] The direct immunofluorescence assay developed in this study was rapid, specific and convenient, and had great significance for the early diagnosis of canine distemper. |
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| Keywords: | Canine distemper virus Direct immunofluorescence assay Monoclonal antibody |
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