紫鸭跖草细胞中铜的分配和化学形态特征研究(英文)

Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.Method]The distribution characteristics of Cu2+ in subcells of Setcreasea purpurea Boom was studied by the technique of differential centrifugation,and the binding form of Cu2+ in roots and leaves of Setcreasea purpurea Boom was also investigated by the sequential chemical extraction method and the enzymolysis method.Result]Cu2+ in roots mainly distributed in the cell wall which is accounting for one third of the Total Cu2+ in roots,while Cu2+ in leaves mainly distributed in the chloroplast which is accounting for a quarter to the Total Cu2+ in leaves.Under the high concentration of Cu2+ or the extended treatment duration,the translocation of Cu2+ in root cells into the cell wall increased but the translocation of Cu2+ in root cells into the plastid decreased,while the translocation of Cu2+ in leaf cells into the chloroplast increased but the translocation of Cu2+ in leaf cells into the cell wall decreased.Cu2+ in leaves was mainly combined with amino acid,small molecular polymeric pigments,protein and polysaccharide,while Cu2+ in roots was mainly combined with cell wall substances such as cellulose and membrane-bound protein.Conclusion]The distribution characteristics and binding form of Cu2+ in cells is possibly one of the dominant mechanisms for Cu2+ hyper-accumulator in Setcreasea purpurea Boom.

Studies on Distribution and Chemical Characteristics of Copper in Cells of Setcreasea purpurea Boom

Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distri-bution characteristics and binding form of Cu2+ in tissue cells. Method] The distribution characteristics of Cu2+ in subcells of Setcreasea purpurea Boomwas studied by the technique of differential centrifugation, and the binding form of Cu2+ in roots and leaves of Setcreasea purpurea Boom was also investiga-ted by the sequential chemical extraction method and the enzymolysis method. Result] Cu2+ in roots mainly distributed in the cell wall which is accounting for one third of the Total Cu2+ in roots, while Cu2+ in leaves mainly distributed in the chloroplast which is accounting for a quarter to the Total Cu2+ in leaves. Under the high concentration of Cu2+ or the extended treatment duration, the translocation of Cu2+ in root cells into the cell wall increased but the translocation of Cu2+ in root cells into the plastid decreased, while the translocation of Cu2+ in leaf cells into the chloroplast increased but the trans-location of Cu2+ in leaf cells into the cell wall decreased. Cu2+ in leaves was mainly combined with amino acid, small molecular polymeric pigments, pro-tein and polysaccharide, while Cu2+ in roots was mainly combined with cell wail substances such as cellulose and membrone-bound protein. Conclusion] The distribution characteristics and binding form of Cu2+ in cells is possibly one of the dominant mechanisms for Cu2+ hyper-accumnlator in Setcreasea pur-purea Boom.