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2,4-D和6-BA对籼稻成熟胚愈伤组织诱导和植株再生的影响
引用本文:苗春波,万志刚,孙丙耀. 2,4-D和6-BA对籼稻成熟胚愈伤组织诱导和植株再生的影响[J]. 农业科学与技术, 2009, 10(4): 22-26
作者姓名:苗春波  万志刚  孙丙耀
作者单位:苏州大学基础医学与生物科学学院,江苏苏州,215123 
基金项目:the National Natural Science Foundation (30571049).国家自然科学基金资助项目 
摘    要:1材料与方法1.1材料供试材料为12个籼稻品种的成熟种子(籽实)。其中,籼小粘、嘉育948、中二软占、湘晚籼1号、遵籼3号、中鉴1006个品种由国家水稻种质资源中期库提供,扬稻6号、扬籼6547、盐恢559、镇恢084、广恢998、明恢86六个品种由江苏太湖农业科学研究所提供。

关 键 词:籼稻品种  愈伤组织诱导  植株再生  6-BA  成熟胚  农业科学研究所  嘉育948  盐恢559

Effects of 2,4-D and 6-BA on Callus Induction and Plantlet Regeneration from Mature Embryos of Hsien Rice
MIAO Chun-bo,WAN Zhi-gang,SUN Bing-yao. Effects of 2,4-D and 6-BA on Callus Induction and Plantlet Regeneration from Mature Embryos of Hsien Rice[J]. Agricultural Science & Technology, 2009, 10(4): 22-26
Authors:MIAO Chun-bo  WAN Zhi-gang  SUN Bing-yao
Affiliation:( School of Preclinical Medicines & Biological Sciences, Suzhou University, Suzhou 215123)
Abstract:[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiation mediums to study their effects on callus induction, seedling formation and regenerated seedlings rooting. [ Result] In the callus induction medium treated with 0.5 mg/L 2,4-D, the callus induction effects on the varieties like Jiayu 948, Yanghui 559, Yangxian 6547, Zhong'erruanzhan, Minghui 86, Guanghui 998 and Zunxian 3 were the best; If 0.2 mg/L 6-BA was added into the callus induction medium containing the optimum level of 2,4-D, there was no obvious effect on induction rate of callus, but the differentiation and seedling of callus were inhibited; If the concentration of 6-BA was reduced appropriately in the differentiation medium, the seedling rate of callus would be not only no decreased but increased, meanwhile the quality of regenerated plants would be improved. [ Conclusion] The study results provided some references for the reasonable uses of 2,4-D and 6-BA in callus culture of hsien rice.
Keywords:Hsien rice  Callus  2,4-D  6-BA
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