菊花白锈病抗性标准及人工离体接种鉴定方法的优化

目的]探讨在可控条件下接种和鉴定菊花白锈病的有效方法。方法]笔者将目测观察与显微镜检方法相结合,制定了菊花白锈病6级抗性鉴定标准,并优化了人工离体接种方法。结果]结果表明,培养瓶内茎段扦插法、培养皿内平铺叶片法均可用于菊花白锈病的离体鉴定;培养瓶内茎段扦插法优于培养皿内平铺叶片法,是最佳的人工离体接种鉴定方法。结论]本研究为安全有效地进行菊花白锈病的相关研究提供科学方法,以便最大程度地控制该病的进一步发生和流行。

Optimization on Identification Standards and Artificial Inoculation Methods In Vitro on Resistance to Chrysanthemum White Rust

Objective] This research aimed at exploring an effective way for inoculation and identification of chrysanthemum white rust under controlled conditions. Method] By combining the observation methods with the naked eye and under optical microscope, we had established the identification standards for chrysanthemum white rust with six classifications and optimized artificial inoculation methods in vitro. Result] The results showed that bottled cuttings identification method and petri dished leaves identification method both can be used for identification in vitro of chrysanthemum white rust, bottled cuttings identification method had shown better effects than petri dished leaves identification method, and was supposed to be best artificial inoculation and identification method in vitro. Conclusion] This research had provided a scientific method for safe and effective researches on chrysanthemum white rust, in order to control the occurrence and diffusion of this quarantine disease.