| E．tenella吉林株单卵囊的分离及PCR鉴定 |
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| 引用本文： | 杨桂连,李建华,张西臣,赵权,宫鹏涛,蔡亚南,张国才.E．tenella吉林株单卵囊的分离及PCR鉴定[J].农业科学与技术,2009,10(3):55-57,62. |
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| 作者姓名： | 杨桂连 李建华 张西臣 赵权 宫鹏涛 蔡亚南 张国才 |
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| 作者单位： | 杨桂连,赵权,YANG Gui-lian,ZHAO Quan(吉林大学畜牧兽医学院,吉林长春130062;吉林农业大学动物科学技术学院,吉林长春130118);李建华,张西臣,宫鹏涛,蔡亚南,张国才,LI Jian-hua,ZHANG Xi-chen,GONG Peng-tao,CAI Ya-nan,ZHANG Guo-cai(吉林大学畜牧兽医学院,吉林长春,130062) |
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| 摘 要： | 1材料与方法1．1材料①试验动物。1日龄海兰公雏，购自长春市北方鸡场。饲养于150℃，消毒1h的隔离器中，自由采食和饮水。饲喂不添加任何抗球虫药的全价饲料，喂前80℃高温消毒30min。②试验虫种。单卵囊分离用虫种，为该实验室从球虫严重临床发病鸡盲肠中分离。③试剂。基因组DNA提取试剂盒购于天根生化科技（北京）有限公司，D12000及PCR相关试剂购自大连宝生物工程有限公司。其他试剂均为国产分析纯。④仪器。
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| 关 键 词： | E.tenella 吉林 株单卵囊 分离 PCR 鉴定 鸡 |
Isolation of E.tenella JL Strain Single-oocyst and PCR Identification |
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| YANG Gui-lian,LI Jian-hua,ZHANG Xi-chen,ZHAO Quan,GONG Peng-tao,CAI Ya-nan,ZHANG Guo-cai.Isolation of E.tenella JL Strain Single-oocyst and PCR Identification[J].Agricultural Science ＆ Technology,2009,10(3):55-57,62. |
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| Authors: | YANG Gui-lian LI Jian-hua ZHANG Xi-chen ZHAO Quan GONG Peng-tao CAI Ya-nan ZHANG Guo-cai |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062; 2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118) |
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| Abstract: | Objective] In order to get a purified strain to carry out the relative molecular biology research about E.tenella. Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens. At the same time, the collected oocysts were identified by PCR method. Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%. The PCR results demonstrated that the single-oocyst strain was E.tenella. Conclusion] The inoculation of single oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty, increased infection rate and provided good materials for biological research of coccidian. |
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| Keywords: | E tenella Single-oocyst Isolation PCR identification |
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