| 从一株革兰氏阳性菌微杆菌中快速高效提取基因组DNA的新方法（摘要） |
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| 引用本文： | 丁娟芳,杨嘉.从一株革兰氏阳性菌微杆菌中快速高效提取基因组DNA的新方法（摘要）[J].农业科学与技术,2010,11(4):69-71. |
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| 作者姓名： | 丁娟芳 杨嘉 |
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| 作者单位： | 丁娟芳(扬州环境资源职业技术学院,江苏扬州,225127);杨嘉(宝应县产品质量监督检验所,江苏扬州,225800) |
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| 摘 要： | 目的]探索可以快速从革兰氏阳性菌微杆菌中提取高质量基因组总DNA的新方法。方法]采用3种不同方法提取微杆菌基因组DNA,并进行DNA纯度、完整度鉴定及16SrDNA扩增检测。结果]紫外吸收值表明,采用改良方法所获DNAA260/A280大于1.80,A260/A230为2.0,5ml过夜菌液可得6.5μg基因组总DNA。DNA凝胶电泳结果表明DNA完整度高且无RNA污染。以此方法提取的基因组总DNA样品为模板,可灵敏有效扩增16SrDNA基因。结论]该方法用时短,操作简易,纯度好、产率高、成本低,适用于革兰氏阳性菌各相关的分子生物学研究。
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| 关 键 词： | 革兰氏阳性菌 微杆菌 基因组DNA提取 DNA质量 16SrDNA |
A Rapid and Efficient Method for Preparing Genomic DNA from Microbacterium sp. |
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| DING Juan-fang,YANG Jia.A Rapid and Efficient Method for Preparing Genomic DNA from Microbacterium sp.[J].Agricultural Science ＆ Technology,2010,11(4):69-71. |
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| Authors: | DING Juan-fang YANG Jia |
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| Institution: | 1.Yangzhou Vocational College of Environment And Resources,Yangzhou 225127;2.Baoying Products Quality Supervision And Inspection Institute,Yangzhou 225800 ) |
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| Abstract: | A new method was used to preparing genomic DNA from Microbacterium sp.quickly and efficiently.DNA quantity and purity was measured by UV absorbance.Integrity of the genomic DNA was tested by agarose gel eletrophoresis.The DNA prepared by this method was sufficiently pure for PCR.This method saves time and cost,practices easily as well. |
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| Keywords: | 16S rDNA Gram-positive bacteria Microbacterium sp Genomic DNA preparation DNA quality 16S rDNA |
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