羊传染性脓疱病毒B2L基因片段重组腺病毒载体的构建（摘要）（英文）

目的]利用腺病毒载体系统构建羊传染性脓疱病毒B2L基因重组腺病毒载体。方法]以从羊传染性脓疱病毒株JLSY04中提取的基因组DNA为模板,PCR扩增获得B2L目的基因片段;然后将B2L目的基因克隆至PD-NR-CMV载体,筛选阳性克隆获得质粒CTC572-6;再将质粒CTC572-6与腺病毒载体进行同源重组,筛选阳性克隆,并进行菌液PCR、酶切、测序等鉴定。结果]经酶切和基因测序等鉴定,成功构建了携带羊传染性脓疱病毒B2L基因的重组腺病毒载体CTC572Ade-30。结论]为羊传染性脓疱基因工程疫苗的进一步研究奠定基础。

Construction of Recombinant Adenovirus Vector Containing CEVB2L Gene

Objective] Sheep contagious ecthyma virus B2L gene recombinant adenovirus was built by adenovirus vector system.Method] Genome DNA extracted from sheep contagious ecthyma virus strain JLSY04 as a template,Gene fragments obtained from B2L by PCR amplification;B2L gene cloning was cloned into PDNR-CMD vector,screening positive clones and plasmid CTC572-6 was obtained;CTC572-6 plasmid for homologous was recombined with the adenoviral vector.Screening positive clones and bacilli PCR,digestion and sequencing and so on were identified.Result] After identified by enzyme digestion and gene sequencing,recombinant adenovirus vector CTC572Ade-30 of carrying sheep contagious ecthyma virus B2L gene was constructed successfully.Conclusion] Which laid the foundation for sheep contagious ecthyma genetically engineered vaccine.