中华绒螯蟹与合浦绒螯蟹线粒体细胞色素b基因片段的DGGE分析

目的]探讨利用DGGE技术对中华绒螯蟹和合浦绒螯蟹遗传多样性进行分析的可能性。方法]对中华绒螯蟹5个群体和合浦绒螯蟹1个群体共180个个体的线粒体细胞色素b（cytb）基因片段进行了PCR扩增和变性梯度凝胶电泳（DGGE）分析。结果]所有PCR产物在DGGE电泳时共有2种迁移速率,合浦绒螯蟹所有个体的PCR产物的迁移速率相同,且中华绒螯蟹江都群体46.7%、仪征群体23.3%以及温州群体20.0%的个体与合浦绒螯蟹的迁移速率一致;而以上3个中华绒螯蟹群体的其他个体以及南京、盘锦群体所有个体的PCR产物在DGGE电泳时的迁移速率均相同,均快于合浦绒螯蟹,表明在中华绒螯蟹中混杂有合浦绒螯蟹的遗传标记类型。结论]DGGE方法可用于绒螯蟹的遗传多样性分析。

DGGE Analysis on Mitochondrial Cyt b Gene of Eriocheir sinensis and Eriocheir hepuensis

Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis(DGGE).Method] Mitochondrial cyt b gene fragment was amplified from 180 individuals of five populations of E.sinensis and a population of E.hepuensis and then analyzed by using DGGE.Result] All PCR products showed two kinds of electrophoretic mobility on DGGE.The PCR products of all individuals from E.hepuensis showed the same mobility with that of the individuals from 46.7% of Jiangdu population,23.3% of Yizheng population and 20.0% of Wenzhou population of E.sinensis,while the rest of the individuals from the three populations of E.sinensis mentioned above as well as all the individuals of Nanjing and Panjin populations showed the same mobility,which was higher compared with that of E.hepuensis.The results indicated that there was the same genetic marker in E.sinensis populations as that of E.hepuensis population,which was consistent with previous studies.Conclusion] DGGE technique could be used to analyze the genetic diversity of Chinese mitten crab.