水果型番木瓜组织培养的研究

目的]研究水果型番木瓜的组织培养。方法]以经预处理的种子为试材,通过MS基本培养基附加不同浓度的6-BA和IBA,对水果型番木瓜组培快繁技术进行了研究。结果]无菌条件下用75%酒精均匀擦拭水果型番木瓜果皮表面,取出种子,用无菌水冲洗3次是较好的灭菌方法,种子污染率仅为2.52%;水果型番木瓜种子经1000mg/LGA3＋1mg/L6-BA（等体积混合）浸泡18h后接种于MS培养基中培养,种子发芽率、胚芽长度、胚根长度和苗高达到较高值,分别为68.42%、2.25、0.80和1.52cm,幼苗整体生长较好;继代增殖培养基为MS＋0.5mg/L6-BA＋0.1mg/LIBA时增殖系数最高（7.92）,幼苗长势较好;组培苗玻璃化随着光照强度的增强而呈减弱趋势,3000lx处理下玻璃化苗比率最低（3.21%）。结论]该研究为水果型番木瓜的组培快繁研究提供了参考。

Tissue Culture of Mini-Papaya

Objective] The aim was to study the tissue culture of mini-Papaya.Method] The pretreatment seeds of mini-Papaya were cultured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.Result] In the condition of aseptic strain,the surface of mini-Papaya peel was uniformly wiped by 75% alcohol,then seeds were removed and washed by aseptic water for 3 times,which was the best sterilization method,and the pollution rate of seed was only 2.52%.After seeds which had been soaked by the equi-volume mix-solution of 1 000 mg/L GA3 and 1 mg/L 6-BA for 18 h were further purified in MS medium,the germination rate of seed,the length of embryo bud and radicle and the height of seedling were 68.42%,2.25,0.80 and 1.52 cm respectively,furthermore the total situation of seedling growth was better.When subculture multiplication medium was MS＋0.5 mg/L 6-BA＋0.1 mg/L IBA medium,proliferation coefficient of subculture multiplication reached the highest （7.92）,and the seedlings grew better.The ratio of vitrified shoots decreasing with the increase of light intensity could reach the lowest level （3.21%） under the light intensity of 3 000 lx.Conclusion] The research provides reference for studies on tissue culture and rapid propagation of mini-Papaya.