GTPase RABE1b参与拟南芥胚胎发育的研究

目的]研究影响拟南芥早期胚胎发育分裂模式的基因。方法]从拟南芥T-DNA插入的突变体库中分离到2个T-DNA插入突变体,通过表型观察胚胎发育情况。结果]PCR-WALKING表明T-DNA插入位点分别位于基因At4g20360的5＇非编码区和启动子区,基因编码的GTPase RABE1b蛋白为Rab蛋白家族的成员,将这2个突变体分别命名为Atrabe1 b-1和Atrabe1b-2。透明分析发现此突变体在胚胎发育早期球形胚时期分裂模式出现异常,RT-PCR分析发现此基因在拟南芥中以组成型表达。结论]基因At4g20360影响拟南芥早期胚胎发育的分裂模式,推测其编码的蛋白GTPaseRABE1b可能对控制拟南芥胚胎发育过程中的细胞分裂起重要作用。

Study on Involvement of the GTPase RABE1b in Embryonic Development of Arabidopsis thaliana

Objective] This study aimed to study the genes affecting division mode in the early embryonic development of Arabidopsis thaliana.Method] Two T-DNA insertion mutants were isolated from the mutant library of A.thaliana inserted by T-DNA,and the embryonic development was reflected by phenotypes.Result] Genomic PCR-WALKING analyses demonstrated that T-DNA insertion sites were in 5’ non-coding region and promoter region of gene At4g20360 which encodes Rab GTPase RABE1b,and the two mutants were named as Atrabe1b-1 and Atrabe1b-2,respectively.Microscopic analysis on mutants revealed that there was an abnormal cell division pattern in early globular stage of embryonic development.In addition,RT-PCR analysis showed that gene At4g20360 was constitutively expressed in A.thaliana.Conclusion] Gene At4g20360 affected the division pattern of early embryonic development in A.thaliana,and encoded protein GTPase RABE1b which may play an important role in cell division during the embryonic development of A.thaliana.