飞扬草化学成分研究(英文)

目的]对飞扬草地上部分进行化学成分研究。方法]利用正反相硅胶和Sephadex LH-20对飞扬草的化学成分进行分离、纯化,并利用MS,NMR等光谱技术鉴定化合物结构,采用流式细胞术对化合物1,4,5,6进行了小鼠脾细胞免疫刺激活性测试。结果]从飞扬草地上部分分离得到6个化合物,经鉴定分别为邻苯二甲酸二异丁基酯(化合物1)、邻苯二甲酸二乙基己基酯(化合物2)、高车前素(化合物3)、过氧化乙酰(化合物4)、槲皮素(化合物5)和没食子酸(化合物6),所有测试化合物均未发现有明显的免疫刺激活性。结论]成分1~3为首次从飞扬草中分离得到,4为首次从天然产物中得到。该研究为从飞扬草中寻找生物活性物质提供了参考依据。

Studies on the Chemical Constituents of Euphorbia hirta

Objective] The aim was to study the chemical constituents of Euphorbia hirta. Method] The constituents were isolated and purified by various chromatography methods including normal phase and reverse phase silica gel as well as Sephadex LH-20 gel column, and then elucidated by spectroscopic analysis such as MS and NMR etc. Compounds 1, 4, 5 and 6 were evaluated for their immunostimulatory activity against splenic cell of mouse by FCM. Result] Six compounds were isolated from Euphorbia hirt, and identified as diisobutyl-O-phthalate (compond 1), diethylhexyl phthalate (compond 2), hispidulin (compond 3), acetyl peroxide (compond 4), quercetein (compond 5) and gallic acid (compond 6). All compounds were inactive against splenic cell of mouse. Conclusion] Compounds 1, 2 and 3 were isolated from this plant for the first time, and compound 4 was the first naturally occurring compound. This study provided reference for isolating bioactive substances from Euphorbia hirta.